Please use this identifier to cite or link to this item: https://doi.org/10.7554/eLife.33555
Title: Vibrator and PI4KIII? govern neuroblast polarity by anchoring non-muscle myosin II
Authors: Koe C.T. 
Tan Y.S. 
Lönnfors M.
Hur S.K.
Low C.S.L. 
Zhang Y. 
Kanchanawong P. 
Bankaitis V.A.
Wang H. 
Keywords: arsenosobenzene
carrier protein
lipid binding protein
myosin II
phosphatidylinositol 4 phosphate
phosphatidylinositol kinase
Drosophila protein
minor histocompatibility antigen
myosin II
phosphatidylinositol phosphate 4-kinase
phospholipid transfer protein
phosphotransferase
protein binding
Sqh protein, Drosophila
Vib protein, Drosophila
animal cell
Article
binding assay
brain asymmetry
cell division
cell membrane
cell polarity
clone
homeostasis
immunoblotting
immunohistochemistry
in vivo study
light chain
mitosis spindle
molecular cloning
neuroblast
nonhuman
nucleolus
protein lipid interaction
sequence alignment
stem cell
transgenic fish
animal
brain
Drosophila
growth, development and aging
larva
metabolism
neural stem cell
physiology
Animals
Brain
Cell Polarity
Drosophila
Drosophila Proteins
Larva
Minor Histocompatibility Antigens
Myosin Type II
Neural Stem Cells
Phospholipid Transfer Proteins
Phosphotransferases (Alcohol Group Acceptor)
Protein Binding
Issue Date: 2018
Citation: Koe C.T., Tan Y.S., Lönnfors M., Hur S.K., Low C.S.L., Zhang Y., Kanchanawong P., Bankaitis V.A., Wang H. (2018). Vibrator and PI4KIII? govern neuroblast polarity by anchoring non-muscle myosin II. eLife 7 : e33555. ScholarBank@NUS Repository. https://doi.org/10.7554/eLife.33555
Abstract: A central feature of most stem cells is the ability to self-renew and undergo differentiation via asymmetric division. However, during asymmetric division the role of phosphatidylinositol (PI) lipids and their regulators is not well established. Here, we show that the sole type I PI transfer protein, Vibrator, controls asymmetric division of Drosophilaneural stem cells (NSCs) by physically anchoring myosin II regulatory light chain, Sqh, to the NSC cortex. Depletion of vib or disruption of its lipid binding and transfer activities disrupts NSC polarity. We propose that Vib stimulates PI4KIIIa to promote synthesis of a plasma membrane pool of phosphatidylinositol 4-phosphate [PI(4)P] that, in turn, binds and anchors myosin to the NSC cortex. Remarkably, Sqh also binds to PI(4)P in vitro and both Vib and Sqh mediate plasma membrane localization of PI(4)P in NSCs. Thus, reciprocal regulation between Myosin and PI(4)P likely governs asymmetric division of NSCs. © Koe et al.
Source Title: eLife
URI: https://scholarbank.nus.edu.sg/handle/10635/175067
ISSN: 2050084X
DOI: 10.7554/eLife.33555
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