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https://doi.org/10.7554/eLife.33555
Title: | Vibrator and PI4KIII? govern neuroblast polarity by anchoring non-muscle myosin II | Authors: | Koe C.T. Tan Y.S. Lönnfors M. Hur S.K. Low C.S.L. Zhang Y. Kanchanawong P. Bankaitis V.A. Wang H. |
Keywords: | arsenosobenzene carrier protein lipid binding protein myosin II phosphatidylinositol 4 phosphate phosphatidylinositol kinase Drosophila protein minor histocompatibility antigen myosin II phosphatidylinositol phosphate 4-kinase phospholipid transfer protein phosphotransferase protein binding Sqh protein, Drosophila Vib protein, Drosophila animal cell Article binding assay brain asymmetry cell division cell membrane cell polarity clone homeostasis immunoblotting immunohistochemistry in vivo study light chain mitosis spindle molecular cloning neuroblast nonhuman nucleolus protein lipid interaction sequence alignment stem cell transgenic fish animal brain Drosophila growth, development and aging larva metabolism neural stem cell physiology Animals Brain Cell Polarity Drosophila Drosophila Proteins Larva Minor Histocompatibility Antigens Myosin Type II Neural Stem Cells Phospholipid Transfer Proteins Phosphotransferases (Alcohol Group Acceptor) Protein Binding |
Issue Date: | 2018 | Citation: | Koe C.T., Tan Y.S., Lönnfors M., Hur S.K., Low C.S.L., Zhang Y., Kanchanawong P., Bankaitis V.A., Wang H. (2018). Vibrator and PI4KIII? govern neuroblast polarity by anchoring non-muscle myosin II. eLife 7 : e33555. ScholarBank@NUS Repository. https://doi.org/10.7554/eLife.33555 | Abstract: | A central feature of most stem cells is the ability to self-renew and undergo differentiation via asymmetric division. However, during asymmetric division the role of phosphatidylinositol (PI) lipids and their regulators is not well established. Here, we show that the sole type I PI transfer protein, Vibrator, controls asymmetric division of Drosophilaneural stem cells (NSCs) by physically anchoring myosin II regulatory light chain, Sqh, to the NSC cortex. Depletion of vib or disruption of its lipid binding and transfer activities disrupts NSC polarity. We propose that Vib stimulates PI4KIIIa to promote synthesis of a plasma membrane pool of phosphatidylinositol 4-phosphate [PI(4)P] that, in turn, binds and anchors myosin to the NSC cortex. Remarkably, Sqh also binds to PI(4)P in vitro and both Vib and Sqh mediate plasma membrane localization of PI(4)P in NSCs. Thus, reciprocal regulation between Myosin and PI(4)P likely governs asymmetric division of NSCs. © Koe et al. | Source Title: | eLife | URI: | https://scholarbank.nus.edu.sg/handle/10635/175067 | ISSN: | 2050084X | DOI: | 10.7554/eLife.33555 |
Appears in Collections: | Elements Staff Publications |
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