Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41598-018-20304-5
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dc.titleDirected Differentiation of Adult Liver Derived Mesenchymal Like Stem Cells into Functional Hepatocytes
dc.contributor.authorLuo X.
dc.contributor.authorGupta K.
dc.contributor.authorAnanthanarayanan A.
dc.contributor.authorWang Z.
dc.contributor.authorXia L.
dc.contributor.authorLi A.
dc.contributor.authorSakban R.B.
dc.contributor.authorLiu S.
dc.contributor.authorYu H.
dc.date.accessioned2020-09-09T03:04:43Z
dc.date.available2020-09-09T03:04:43Z
dc.date.issued2018
dc.identifier.citationLuo X., Gupta K., Ananthanarayanan A., Wang Z., Xia L., Li A., Sakban R.B., Liu S., Yu H. (2018). Directed Differentiation of Adult Liver Derived Mesenchymal Like Stem Cells into Functional Hepatocytes. Scientific Reports 8 (1) : 2818. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-018-20304-5
dc.identifier.issn20452322
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/175029
dc.description.abstractShortage of functional hepatocytes hampers drug safety testing and therapeutic applications because mature hepatocytes cannot be expanded and maintain functions in vitro. Recent studies have reported that liver progenitor cells can originate from mature hepatocytes in vivo. Derivation of proliferating progenitor cells from mature hepatocytes, and re-differentiation into functional hepatocytes in vitro has not been successful. Here we report the derivation of novel mesenchymal-like stem cells (arHMSCs) from adult rat hepatocytes. Immunofluorescence and flow cytometry characterization of arHMSCs found expression of mesenchymal markers CD29, CD44, CD90, vimentin and alpha smooth muscle actin. These arHMSCs proliferated in vitro for 4 passages yielding 104 fold increase in cell number in 28 days, and differentiated into hepatocyte-like cells (arHMSC-H). The arHMSC-H expressed significantly higher level of hepatocyte-specific markers (200 fold for albumin and 6 fold for Cyp450 enzymes) than arHMSCs. The arHMSC-H also demonstrated dose response curves similar to primary hepatocytes for 3 of the 6 paradigm hepatotoxicants tested, demonstrating utility in drug safety testing applications. © 2018 The Author(s).
dc.sourceUnpaywall 20200831
dc.subjectbiological marker
dc.subjectanimal
dc.subjectcell culture
dc.subjectcell culture technique
dc.subjectcell differentiation
dc.subjectcell proliferation
dc.subjectcytology
dc.subjectflow cytometry
dc.subjectliver
dc.subjectliver cell
dc.subjectmale
dc.subjectmesenchymal stem cell
dc.subjectmetabolism
dc.subjectphysiology
dc.subjectprocedures
dc.subjectrat
dc.subjectstem cell
dc.subjectWistar rat
dc.subjectAnimals
dc.subjectBiomarkers
dc.subjectCell Culture Techniques
dc.subjectCell Differentiation
dc.subjectCell Proliferation
dc.subjectCells, Cultured
dc.subjectFlow Cytometry
dc.subjectHepatocytes
dc.subjectLiver
dc.subjectMale
dc.subjectMesenchymal Stem Cells
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectStem Cells
dc.typeArticle
dc.contributor.departmentMECHANOBIOLOGY INSTITUTE
dc.contributor.departmentDEPT OF PHYSIOLOGY
dc.description.doi10.1038/s41598-018-20304-5
dc.description.sourcetitleScientific Reports
dc.description.volume8
dc.description.issue1
dc.description.page2818
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