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https://doi.org/10.18632/oncotarget.2291
Title: | A central role for TRPS1 in the control of cell cycle and cancer development | Authors: | Wu, L Wang, Y Liu, Y Yu, S Xie, H Shi, X Qin, S Ma, F Tan, T.Z Thiery, J.P Chen, L |
Keywords: | histone deacetylase 2 histone deacetylase 4 protein TRPS1 transcription factor unclassified drug DNA binding protein small interfering RNA transcription factor TRPS1 protein, human Article breast cancer cancer cell line carcinogenesis cell cycle G2 phase cell cycle M phase cell cycle progression cell cycle regulation cell proliferation cell synchronization controlled study genetic regulation histone acetylation human human cell protein expression protein function apoptosis breast tumor cell cycle chromatin immunoprecipitation DNA microarray fluorescent antibody technique gene expression regulation genetics immunoprecipitation pathology real time polymerase chain reaction tumor cell line Western blotting Apoptosis Blotting, Western Breast Neoplasms Cell Cycle Cell Line, Tumor Chromatin Immunoprecipitation DNA-Binding Proteins Fluorescent Antibody Technique Gene Expression Regulation, Neoplastic Humans Immunoprecipitation Oligonucleotide Array Sequence Analysis Real-Time Polymerase Chain Reaction RNA, Small Interfering Transcription Factors |
Issue Date: | 2014 | Publisher: | Impact Journals LLC | Citation: | Wu, L, Wang, Y, Liu, Y, Yu, S, Xie, H, Shi, X, Qin, S, Ma, F, Tan, T.Z, Thiery, J.P, Chen, L (2014). A central role for TRPS1 in the control of cell cycle and cancer development. Oncotarget 5 (17) : 7677-7690. ScholarBank@NUS Repository. https://doi.org/10.18632/oncotarget.2291 | Abstract: | The eukaryotic cell cycle is controlled by a complex regulatory network, which is still poorly understood. Here we demonstrate that TRPS1, an atypical GATA factor, modulates cell proliferation and controls cell cycle progression. Silencing TRPS1 had a differential effect on the expression of nine key cell cycle-related genes. Eight of these genes are known to be involved in the regulation of the G2 phase and the G2/M transition of the cell cycle. Using cell synchronization studies, we confirmed that TRPS1 plays an important role in the control of cells in these phases of the cell cycle. We also show that silencing TRPS1 controls the expression of 53BP1, but not TP53. TRPS1 silencing also decreases the expression of two histone deacetylases, HDAC2 and HDAC4, as well as the overall HDAC activity in the cells, and leads to the subsequent increase in the acetylation of histone4 K16 but not of histone3 K9 or K18. Finally, we demonstrate that TRPS1 expression is elevated in luminal breast cancer cells and luminal breast cancer tissues as compared with other breast cancer subtypes. Overall, our study proposes that TRPS1 acts as a central hub in the control of cell cycle and proliferation during cancer development. | Source Title: | Oncotarget | URI: | https://scholarbank.nus.edu.sg/handle/10635/174998 | ISSN: | 19492553 | DOI: | 10.18632/oncotarget.2291 |
Appears in Collections: | Elements Staff Publications |
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