Please use this identifier to cite or link to this item: https://doi.org/10.1038/ncomms10968
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dc.titleAcetylation of C/EBP? inhibits its granulopoietic function
dc.contributor.authorBararia, D
dc.contributor.authorKwok, H.S
dc.contributor.authorWelner, R.S
dc.contributor.authorNumata, A
dc.contributor.authorSárosi, M.B
dc.contributor.authorYang, H
dc.contributor.authorWee, S
dc.contributor.authorTschuri, S
dc.contributor.authorRay, D
dc.contributor.authorWeigert, O
dc.contributor.authorLevantini, E
dc.contributor.authorEbralidze, A.K
dc.contributor.authorGunaratne, J
dc.contributor.authorTenen, D.G
dc.date.accessioned2020-09-09T01:39:34Z
dc.date.available2020-09-09T01:39:34Z
dc.date.issued2016
dc.identifier.citationBararia, D, Kwok, H.S, Welner, R.S, Numata, A, Sárosi, M.B, Yang, H, Wee, S, Tschuri, S, Ray, D, Weigert, O, Levantini, E, Ebralidze, A.K, Gunaratne, J, Tenen, D.G (2016). Acetylation of C/EBP? inhibits its granulopoietic function. Nature Communications 7 : 10968. ScholarBank@NUS Repository. https://doi.org/10.1038/ncomms10968
dc.identifier.issn20411723
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/174982
dc.description.abstractCCAAT/enhancer-binding protein alpha (C/EBP?) is an essential transcription factor for myeloid lineage commitment. Here we demonstrate that acetylation of C/EBP? at lysine residues K298 and K302, mediated at least in part by general control non-derepressible 5 (GCN5), impairs C/EBP? DNA-binding ability and modulates C/EBP? transcriptional activity. Acetylated C/EBP? is enriched in human myeloid leukaemia cell lines and acute myeloid leukaemia (AML) samples, and downregulated upon granulocyte-colony stimulating factor (G-CSF)- mediated granulocytic differentiation of 32Dcl3 cells. C/EBP? mutants that mimic acetylation failed to induce granulocytic differentiation in C/EBP?-dependent assays, in both cell lines and in primary hematopoietic cells. Our data uncover GCN5 as a negative regulator of C/EBP? and demonstrate the importance of C/EBP? acetylation in myeloid differentiation.
dc.publisherNature Publishing Group
dc.sourceUnpaywall 20200831
dc.subjectCCAAT enhancer binding protein alpha
dc.subjectgranulocyte colony stimulating factor
dc.subjecthistone acetyltransferase GCN5
dc.subjectlysine
dc.subjectCCAAT enhancer binding protein alpha
dc.subjectgranulocyte colony stimulating factor
dc.subjecthistone acetyltransferase PCAF
dc.subjectp300-CBP-associated factor
dc.subjectacetylation
dc.subjectacute myeloblastic leukemia
dc.subjectArticle
dc.subjectbone marrow cell
dc.subjectcell differentiation
dc.subjectcontrolled study
dc.subjectDNA binding
dc.subjectdown regulation
dc.subjectgranulopoiesis
dc.subjecthuman
dc.subjecthuman cell
dc.subjectleukemia cell
dc.subjectmyeloid leukemia
dc.subjectmyeloid leukemia cell line
dc.subjectacetylation
dc.subjectacute myeloid leukemia
dc.subjectcytology
dc.subjectgel mobility shift assay
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectgranulocyte
dc.subjectHEK293 cell line
dc.subjectimmunoblotting
dc.subjectimmunoprecipitation
dc.subjectliquid chromatography
dc.subjectmass spectrometry
dc.subjectmetabolism
dc.subjectmyelopoiesis
dc.subjecttumor cell line
dc.subjectAcetylation
dc.subjectCCAAT-Enhancer-Binding Protein-alpha
dc.subjectCell Differentiation
dc.subjectCell Line, Tumor
dc.subjectChromatography, Liquid
dc.subjectElectrophoretic Mobility Shift Assay
dc.subjectGene Expression Regulation, Neoplastic
dc.subjectGranulocyte Colony-Stimulating Factor
dc.subjectGranulocytes
dc.subjectHEK293 Cells
dc.subjectHumans
dc.subjectImmunoblotting
dc.subjectImmunoprecipitation
dc.subjectLeukemia, Myeloid
dc.subjectLeukemia, Myeloid, Acute
dc.subjectMass Spectrometry
dc.subjectMyelopoiesis
dc.subjectp300-CBP Transcription Factors
dc.typeArticle
dc.contributor.departmentCANCER SCIENCE INSTITUTE OF SINGAPORE
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.contributor.departmentANATOMY
dc.contributor.departmentMEDICINE
dc.description.doi10.1038/ncomms10968
dc.description.sourcetitleNature Communications
dc.description.volume7
dc.description.page10968
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