Please use this identifier to cite or link to this item:
https://doi.org/10.1038/ncomms10968
DC Field | Value | |
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dc.title | Acetylation of C/EBP? inhibits its granulopoietic function | |
dc.contributor.author | Bararia, D | |
dc.contributor.author | Kwok, H.S | |
dc.contributor.author | Welner, R.S | |
dc.contributor.author | Numata, A | |
dc.contributor.author | Sárosi, M.B | |
dc.contributor.author | Yang, H | |
dc.contributor.author | Wee, S | |
dc.contributor.author | Tschuri, S | |
dc.contributor.author | Ray, D | |
dc.contributor.author | Weigert, O | |
dc.contributor.author | Levantini, E | |
dc.contributor.author | Ebralidze, A.K | |
dc.contributor.author | Gunaratne, J | |
dc.contributor.author | Tenen, D.G | |
dc.date.accessioned | 2020-09-09T01:39:34Z | |
dc.date.available | 2020-09-09T01:39:34Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Bararia, D, Kwok, H.S, Welner, R.S, Numata, A, Sárosi, M.B, Yang, H, Wee, S, Tschuri, S, Ray, D, Weigert, O, Levantini, E, Ebralidze, A.K, Gunaratne, J, Tenen, D.G (2016). Acetylation of C/EBP? inhibits its granulopoietic function. Nature Communications 7 : 10968. ScholarBank@NUS Repository. https://doi.org/10.1038/ncomms10968 | |
dc.identifier.issn | 20411723 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/174982 | |
dc.description.abstract | CCAAT/enhancer-binding protein alpha (C/EBP?) is an essential transcription factor for myeloid lineage commitment. Here we demonstrate that acetylation of C/EBP? at lysine residues K298 and K302, mediated at least in part by general control non-derepressible 5 (GCN5), impairs C/EBP? DNA-binding ability and modulates C/EBP? transcriptional activity. Acetylated C/EBP? is enriched in human myeloid leukaemia cell lines and acute myeloid leukaemia (AML) samples, and downregulated upon granulocyte-colony stimulating factor (G-CSF)- mediated granulocytic differentiation of 32Dcl3 cells. C/EBP? mutants that mimic acetylation failed to induce granulocytic differentiation in C/EBP?-dependent assays, in both cell lines and in primary hematopoietic cells. Our data uncover GCN5 as a negative regulator of C/EBP? and demonstrate the importance of C/EBP? acetylation in myeloid differentiation. | |
dc.publisher | Nature Publishing Group | |
dc.source | Unpaywall 20200831 | |
dc.subject | CCAAT enhancer binding protein alpha | |
dc.subject | granulocyte colony stimulating factor | |
dc.subject | histone acetyltransferase GCN5 | |
dc.subject | lysine | |
dc.subject | CCAAT enhancer binding protein alpha | |
dc.subject | granulocyte colony stimulating factor | |
dc.subject | histone acetyltransferase PCAF | |
dc.subject | p300-CBP-associated factor | |
dc.subject | acetylation | |
dc.subject | acute myeloblastic leukemia | |
dc.subject | Article | |
dc.subject | bone marrow cell | |
dc.subject | cell differentiation | |
dc.subject | controlled study | |
dc.subject | DNA binding | |
dc.subject | down regulation | |
dc.subject | granulopoiesis | |
dc.subject | human | |
dc.subject | human cell | |
dc.subject | leukemia cell | |
dc.subject | myeloid leukemia | |
dc.subject | myeloid leukemia cell line | |
dc.subject | acetylation | |
dc.subject | acute myeloid leukemia | |
dc.subject | cytology | |
dc.subject | gel mobility shift assay | |
dc.subject | gene expression regulation | |
dc.subject | genetics | |
dc.subject | granulocyte | |
dc.subject | HEK293 cell line | |
dc.subject | immunoblotting | |
dc.subject | immunoprecipitation | |
dc.subject | liquid chromatography | |
dc.subject | mass spectrometry | |
dc.subject | metabolism | |
dc.subject | myelopoiesis | |
dc.subject | tumor cell line | |
dc.subject | Acetylation | |
dc.subject | CCAAT-Enhancer-Binding Protein-alpha | |
dc.subject | Cell Differentiation | |
dc.subject | Cell Line, Tumor | |
dc.subject | Chromatography, Liquid | |
dc.subject | Electrophoretic Mobility Shift Assay | |
dc.subject | Gene Expression Regulation, Neoplastic | |
dc.subject | Granulocyte Colony-Stimulating Factor | |
dc.subject | Granulocytes | |
dc.subject | HEK293 Cells | |
dc.subject | Humans | |
dc.subject | Immunoblotting | |
dc.subject | Immunoprecipitation | |
dc.subject | Leukemia, Myeloid | |
dc.subject | Leukemia, Myeloid, Acute | |
dc.subject | Mass Spectrometry | |
dc.subject | Myelopoiesis | |
dc.subject | p300-CBP Transcription Factors | |
dc.type | Article | |
dc.contributor.department | CANCER SCIENCE INSTITUTE OF SINGAPORE | |
dc.contributor.department | DUKE-NUS MEDICAL SCHOOL | |
dc.contributor.department | ANATOMY | |
dc.contributor.department | MEDICINE | |
dc.description.doi | 10.1038/ncomms10968 | |
dc.description.sourcetitle | Nature Communications | |
dc.description.volume | 7 | |
dc.description.page | 10968 | |
Appears in Collections: | Elements Staff Publications |
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