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https://doi.org/10.1038/ncomms10968
| Title: | Acetylation of C/EBP? inhibits its granulopoietic function | Authors: | Bararia, D Kwok, H.S Welner, R.S Numata, A Sárosi, M.B Yang, H Wee, S Tschuri, S Ray, D Weigert, O Levantini, E Ebralidze, A.K Gunaratne, J Tenen, D.G |
Keywords: | CCAAT enhancer binding protein alpha granulocyte colony stimulating factor histone acetyltransferase GCN5 lysine CCAAT enhancer binding protein alpha granulocyte colony stimulating factor histone acetyltransferase PCAF p300-CBP-associated factor acetylation acute myeloblastic leukemia Article bone marrow cell cell differentiation controlled study DNA binding down regulation granulopoiesis human human cell leukemia cell myeloid leukemia myeloid leukemia cell line acetylation acute myeloid leukemia cytology gel mobility shift assay gene expression regulation genetics granulocyte HEK293 cell line immunoblotting immunoprecipitation liquid chromatography mass spectrometry metabolism myelopoiesis tumor cell line Acetylation CCAAT-Enhancer-Binding Protein-alpha Cell Differentiation Cell Line, Tumor Chromatography, Liquid Electrophoretic Mobility Shift Assay Gene Expression Regulation, Neoplastic Granulocyte Colony-Stimulating Factor Granulocytes HEK293 Cells Humans Immunoblotting Immunoprecipitation Leukemia, Myeloid Leukemia, Myeloid, Acute Mass Spectrometry Myelopoiesis p300-CBP Transcription Factors |
Issue Date: | 2016 | Publisher: | Nature Publishing Group | Citation: | Bararia, D, Kwok, H.S, Welner, R.S, Numata, A, Sárosi, M.B, Yang, H, Wee, S, Tschuri, S, Ray, D, Weigert, O, Levantini, E, Ebralidze, A.K, Gunaratne, J, Tenen, D.G (2016). Acetylation of C/EBP? inhibits its granulopoietic function. Nature Communications 7 : 10968. ScholarBank@NUS Repository. https://doi.org/10.1038/ncomms10968 | Abstract: | CCAAT/enhancer-binding protein alpha (C/EBP?) is an essential transcription factor for myeloid lineage commitment. Here we demonstrate that acetylation of C/EBP? at lysine residues K298 and K302, mediated at least in part by general control non-derepressible 5 (GCN5), impairs C/EBP? DNA-binding ability and modulates C/EBP? transcriptional activity. Acetylated C/EBP? is enriched in human myeloid leukaemia cell lines and acute myeloid leukaemia (AML) samples, and downregulated upon granulocyte-colony stimulating factor (G-CSF)- mediated granulocytic differentiation of 32Dcl3 cells. C/EBP? mutants that mimic acetylation failed to induce granulocytic differentiation in C/EBP?-dependent assays, in both cell lines and in primary hematopoietic cells. Our data uncover GCN5 as a negative regulator of C/EBP? and demonstrate the importance of C/EBP? acetylation in myeloid differentiation. | Source Title: | Nature Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/174982 | ISSN: | 20411723 | DOI: | 10.1038/ncomms10968 |
| Appears in Collections: | Elements Staff Publications |
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