Please use this identifier to cite or link to this item: https://doi.org/10.18632/oncotarget.8781
Title: Human growth hormone and human prolactin function as autocrine/paracrine promoters of progression of hepatocellular carcinoma
Authors: Kong, X
Wu, W
Yuan, Y
Pandey, V 
Wu, Z
Lu, X
Zhang, W
Chen, Y
Wu, M
Zhang, M
Li, G
Tan, S
Qian, P
Perry, J.K
Lobie, P.E 
Zhu, T
Keywords: human growth hormone
messenger RNA
prolactin
STAT3 protein
human growth hormone
prolactin
adverse outcome
animal experiment
animal model
Article
autocrine effect
cancer grading
cancer growth
cancer patient
cancer size
cancer survival
carcinogenicity
carcinoma cell line
controlled study
gene expression
hGH gene
histopathology
hormone action
hPRL gene
human
human cell
human tissue
immunohistochemistry
in situ hybridization
in vitro study
in vivo study
liver carcinogenesis
liver cell carcinoma
mouse
nonhuman
oncological parameters
outcome assessment
overall survival
paracrine signaling
polymerase chain reaction
promoter region
protein expression
recurrence free survival
transcription initiation
animal
autocrine effect
disease exacerbation
Kaplan Meier method
liver cell carcinoma
liver tumor
metabolism
mortality
nude mouse
paracrine signaling
pathology
physiology
prognosis
tumor cell line
xenograft
Animals
Autocrine Communication
Carcinoma, Hepatocellular
Cell Line, Tumor
Disease Progression
Heterografts
Human Growth Hormone
Humans
Kaplan-Meier Estimate
Liver Neoplasms
Mice
Mice, Nude
Paracrine Communication
Prognosis
Prolactin
Issue Date: 2016
Publisher: Impact Journals LLC
Citation: Kong, X, Wu, W, Yuan, Y, Pandey, V, Wu, Z, Lu, X, Zhang, W, Chen, Y, Wu, M, Zhang, M, Li, G, Tan, S, Qian, P, Perry, J.K, Lobie, P.E, Zhu, T (2016). Human growth hormone and human prolactin function as autocrine/paracrine promoters of progression of hepatocellular carcinoma. Oncotarget 7 (20) : 29465-29479. ScholarBank@NUS Repository. https://doi.org/10.18632/oncotarget.8781
Abstract: The death rates of hepatocellular carcinoma (HCC) are extremely high due to the paucity of therapeutic options. Animal models and anecdotal clinical evidence indicate a potential role of hGH and hPRL in HCC. However, the prognostic relevance and the functional role of tumor expression of these hormones in human HCC are not defined. Herein, we analyzed the mRNA and protein expression of hGH and hPRL in histopathological samples of non-neoplastic liver and HCC by in situ hybridization, PCR and immunohistochemistry techniques. Increased mRNA and protein expression of both hormones was observed in HCC compared with non-neoplastic liver tissues. hGH expression was significantly associated with tumor size and tumor grade. No significant association was observed between the expression of hPRL and any histopathological features. Amplification of both hGH and hPRL genes in HCC was observed when compared to non-neoplastic tissue. Expression of both hGH and hPRL was associated with worse relapse-free and overall survival in HCC patients. In vitro and in vivo functional assays performed with HCC cell lines demonstrated that autocrine expression of hGH or hPRL in HCC cells increased STAT3 activation, oncogenicity and tumor growth while functional antagonism with hGH-G120R significantly reduced these parameters. Hence, tumor expression of hGH/hPRL is associated with a worse survival outcome for patients with HCC and hGH/hPRL function as autocrine/paracrine promoters of HCC progression.
Source Title: Oncotarget
URI: https://scholarbank.nus.edu.sg/handle/10635/174964
ISSN: 19492553
DOI: 10.18632/oncotarget.8781
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