Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep28317
Title: Dazl is a critical player for primordial germ cell formation in medaka
Authors: Li, M 
Zhu, F 
Li, Z 
Hong, N 
Hong, Y 
Keywords: fish protein
RNA binding protein
animal
cytology
embryology
gene knockdown
genetics
germ cell
metabolism
Oryzias
Animals
Fish Proteins
Gene Knockdown Techniques
Germ Cells
Oryzias
RNA-Binding Proteins
Issue Date: 2016
Publisher: Nature Publishing Group
Citation: Li, M, Zhu, F, Li, Z, Hong, N, Hong, Y (2016). Dazl is a critical player for primordial germ cell formation in medaka. Scientific Reports 6 : 28317. ScholarBank@NUS Repository. https://doi.org/10.1038/srep28317
Abstract: The DAZ family genes boule, daz and dazl have conserved functions in primordial germ cell (PGC) migration, germ stem cell proliferation, differentiation and meiosis progression. It has remained unknown whether this family is required for PGC formation in developing embryos. Our recent study in the fish medaka (Oryzias latipes) has defined dnd as the critical PGC specifier and predicted the presence of additional factors essential for PGC formation. Here we report that dazl is a second key player for medaka PGC formation. Dazl knockdown did not prevent PGC formation even in the absence of normal somatic structures. It turned out that a high level of Dazl protein was maternally supplied and persisted until gastrulation, and hardly affected by two antisense morpholino oligos targeting the dazl RNA translation. Importantly, microinjection of a Dazl antibody remarkably reduced the number of PGCs and even completely abolished PGC formation without causing detectable somatic abnormality. Therefore, medaka PGC formation requires the Dazl protein as maternal germ plasm component, offering first evidence that dazl is a critical player in PGC formation in vivo. Our results demonstrate that antibody neutralization is a powerful tool to study the roles of maternal protein factors in PGC development in vivo.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/174952
ISSN: 20452322
DOI: 10.1038/srep28317
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