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https://doi.org/10.1038/ncomms13302
Title: | TRNA-mediated codon-biased translation in mycobacterial hypoxic persistence | Authors: | Chionh, Y.H McBee, M Babu, I.R Hia, F Lin, W Zhao, W Cao, J Dziergowska, A Malkiewicz, A Begley, T.J Alonso, S Dedon, P.C |
Keywords: | heat shock protein messenger RNA ribonucleoside transfer RNA bacterial protein bacterial RNA transcriptome transfer RNA amino acid bacterium gene expression hypoxia molecular analysis pathogen protein RNA tuberculosis Article bacterial survival bacteriostasis cell reprogramming technique codon codon usage environmental change gene expression granuloma hypoxia Mycobacterium bovis BCG nonhuman RNA translation gene expression regulation genetics metabolism Mycobacterium bovis oxygen consumption physiology protein processing protein synthesis Bacteria (microorganisms) Corynebacterineae Mycobacterium bovis BCG Bacterial Proteins Codon Gene Expression Regulation, Bacterial Mycobacterium bovis Oxygen Consumption Protein Biosynthesis Protein Processing, Post-Translational RNA, Bacterial RNA, Messenger RNA, Transfer Transcriptome |
Issue Date: | 2016 | Publisher: | Nature Publishing Group | Citation: | Chionh, Y.H, McBee, M, Babu, I.R, Hia, F, Lin, W, Zhao, W, Cao, J, Dziergowska, A, Malkiewicz, A, Begley, T.J, Alonso, S, Dedon, P.C (2016). TRNA-mediated codon-biased translation in mycobacterial hypoxic persistence. Nature Communications 7 : 13302. ScholarBank@NUS Repository. https://doi.org/10.1038/ncomms13302 | Abstract: | Microbial pathogens adapt to the stress of infection by regulating transcription, translation and protein modification. We report that changes in gene expression in hypoxia-induced non-replicating persistence in mycobacteria - which models tuberculous granulomas - are partly determined by a mechanism of tRNA reprogramming and codon-biased translation. Mycobacterium bovis BCG responded to each stage of hypoxia and aerobic resuscitation by uniquely reprogramming 40 modified ribonucleosides in tRNA, which correlate with selective translation of mRNAs from families of codon-biased persistence genes. For example, early hypoxia increases wobble cmo5U in tRNAThr(UGU), which parallels translation of transcripts enriched in its cognate codon, ACG, including the DosR master regulator of hypoxic bacteriostasis. Codon re-engineering of dosR exaggerates hypoxia-induced changes in codon-biased DosR translation, with altered dosR expression revealing unanticipated effects on bacterial survival during hypoxia. These results reveal a coordinated system of tRNA modifications and translation of codon-biased transcripts that enhance expression of stress response proteins in mycobacteria. © The Author(s) 2016. | Source Title: | Nature Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/174918 | ISSN: | 20411723 | DOI: | 10.1038/ncomms13302 |
Appears in Collections: | Elements Staff Publications |
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