Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep12957
Title: Simultaneous fingerprint and high-wavenumber fiber-optic Raman spectroscopy improves in vivo diagnosis of esophageal squamous cell carcinoma at endoscopy
Authors: Wang J. 
Lin K. 
Zheng W. 
Yu Ho K. 
Teh M. 
Guan Yeoh K. 
Huang Z. 
Keywords: Carcinoma, Squamous Cell
endoscopy
Esophageal Neoplasms
fiber optics
human
metabolism
Raman spectrometry
receiver operating characteristic
Carcinoma, Squamous Cell
Endoscopy
Esophageal Neoplasms
Fiber Optic Technology
Humans
ROC Curve
Spectrum Analysis, Raman
Issue Date: 2015
Citation: Wang J., Lin K., Zheng W., Yu Ho K., Teh M., Guan Yeoh K., Huang Z. (2015). Simultaneous fingerprint and high-wavenumber fiber-optic Raman spectroscopy improves in vivo diagnosis of esophageal squamous cell carcinoma at endoscopy. Scientific Reports 5 : 12957. ScholarBank@NUS Repository. https://doi.org/10.1038/srep12957
Abstract: This work aims to evaluate clinical value of a fiber-optic Raman spectroscopy technique developed for in vivo diagnosis of esophageal squamous cell carcinoma (ESCC) during clinical endoscopy. We have developed a rapid fiber-optic Raman endoscopic system capable of simultaneously acquiring both fingerprint (FP)(800-1800â €‰cm â '1) and high-wavenumber (HW)(2800-3600â €‰cm â '1) Raman spectra from esophageal tissue in vivo. A total of 1172 in vivo FP/HW Raman spectra were acquired from 48 esophageal patients undergoing endoscopic examination. The total Raman dataset was split into two parts: 80% for training; while 20% for testing. Partial least squares-discriminant analysis (PLS-DA) and leave-one patient-out, cross validation (LOPCV) were implemented on training dataset to develop diagnostic algorithms for tissue classification. PLS-DA-LOPCV shows that simultaneous FP/HW Raman spectroscopy on training dataset provides a diagnostic sensitivity of 97.0% and specificity of 97.4% for ESCC classification. Further, the diagnostic algorithm applied to the independent testing dataset based on simultaneous FP/HW Raman technique gives a predictive diagnostic sensitivity of 92.7% and specificity of 93.6% for ESCC identification, which is superior to either FP or HW Raman technique alone. This work demonstrates that the simultaneous FP/HW fiber-optic Raman spectroscopy technique improves real-time in vivo diagnosis of esophageal neoplasia at endoscopy.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/174573
ISSN: 2045-2322
DOI: 10.1038/srep12957
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