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Title: Simultaneous fingerprint and high-wavenumber fiber-optic Raman spectroscopy improves in vivo diagnosis of esophageal squamous cell carcinoma at endoscopy
Authors: Wang J. 
Lin K. 
Zheng W. 
Yu Ho K. 
Teh M. 
Guan Yeoh K. 
Huang Z. 
Keywords: Carcinoma, Squamous Cell
Esophageal Neoplasms
fiber optics
Raman spectrometry
receiver operating characteristic
Carcinoma, Squamous Cell
Esophageal Neoplasms
Fiber Optic Technology
ROC Curve
Spectrum Analysis, Raman
Issue Date: 2015
Citation: Wang J., Lin K., Zheng W., Yu Ho K., Teh M., Guan Yeoh K., Huang Z. (2015). Simultaneous fingerprint and high-wavenumber fiber-optic Raman spectroscopy improves in vivo diagnosis of esophageal squamous cell carcinoma at endoscopy. Scientific Reports 5 : 12957. ScholarBank@NUS Repository.
Abstract: This work aims to evaluate clinical value of a fiber-optic Raman spectroscopy technique developed for in vivo diagnosis of esophageal squamous cell carcinoma (ESCC) during clinical endoscopy. We have developed a rapid fiber-optic Raman endoscopic system capable of simultaneously acquiring both fingerprint (FP)(800-1800â €‰cm â '1) and high-wavenumber (HW)(2800-3600â €‰cm â '1) Raman spectra from esophageal tissue in vivo. A total of 1172 in vivo FP/HW Raman spectra were acquired from 48 esophageal patients undergoing endoscopic examination. The total Raman dataset was split into two parts: 80% for training; while 20% for testing. Partial least squares-discriminant analysis (PLS-DA) and leave-one patient-out, cross validation (LOPCV) were implemented on training dataset to develop diagnostic algorithms for tissue classification. PLS-DA-LOPCV shows that simultaneous FP/HW Raman spectroscopy on training dataset provides a diagnostic sensitivity of 97.0% and specificity of 97.4% for ESCC classification. Further, the diagnostic algorithm applied to the independent testing dataset based on simultaneous FP/HW Raman technique gives a predictive diagnostic sensitivity of 92.7% and specificity of 93.6% for ESCC identification, which is superior to either FP or HW Raman technique alone. This work demonstrates that the simultaneous FP/HW fiber-optic Raman spectroscopy technique improves real-time in vivo diagnosis of esophageal neoplasia at endoscopy.
Source Title: Scientific Reports
ISSN: 2045-2322
DOI: 10.1038/srep12957
Appears in Collections:Staff Publications

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