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https://doi.org/10.1186/1471-2164-11-135
Title: | A consensus linkage map of the grass carp (Ctenopharyngodon idella) based on microsatellites and SNPs | Authors: | Xia, J.H Liu, F Zhu, Z.Y Fu, J Feng, J Li, J Yue, G.H |
Keywords: | animal experiment animal tissue article chromosome number chromosome rearrangement comparative gene mapping Ctenopharyngodon idella Cypriniformes female gender gene locus gene sequence genetic conservation genetic linkage genetic recombination genome analysis grass carp haploidy male microsatellite marker nonhuman nucleotide sequence orthology single nucleotide polymorphism unindexed sequence animal carp chromosome map comparative genomic hybridization DNA sequence gene library genetics genotype methodology quantitative trait locus synteny zebra fish Ctenopharyngodon Ctenopharyngodon idella Cyprinidae Danio rerio microsatellite DNA Animals Carps Chromosome Mapping Comparative Genomic Hybridization Female Genomic Library Genotype Male Microsatellite Repeats Polymorphism, Single Nucleotide Quantitative Trait Loci Sequence Analysis, DNA Synteny Zebrafish |
Issue Date: | 2010 | Publisher: | BioMed Central Ltd. | Citation: | Xia, J.H, Liu, F, Zhu, Z.Y, Fu, J, Feng, J, Li, J, Yue, G.H (2010). A consensus linkage map of the grass carp (Ctenopharyngodon idella) based on microsatellites and SNPs. BMC Genomics 11 (1) : 135. ScholarBank@NUS Repository. https://doi.org/10.1186/1471-2164-11-135 | Abstract: | Background: Grass carp (Ctenopharyngodon idella) belongs to the family Cyprinidae which includes more than 2000 fish species. It is one of the most important freshwater food fish species in world aquaculture. A linkage map is an essential framework for mapping traits of interest and is often the first step towards understanding genome evolution. The aim of this study is to construct a first generation genetic map of grass carp using microsatellites and SNPs to generate a new resource for mapping QTL for economically important traits and to conduct a comparative mapping analysis to shed new insights into the evolution of fish genomes.Results: We constructed a first generation linkage map of grass carp with a mapping panel containing two F1 families including 192 progenies. Sixteen SNPs in genes and 263 microsatellite markers were mapped to twenty-four linkage groups (LGs). The number of LGs was corresponding to the haploid chromosome number of grass carp. The sex-specific map was 1149.4 and 888.8 cM long in females and males respectively whereas the sex-averaged map spanned 1176.1 cM. The average resolution of the map was 4.2 cM/locus. BLAST searches of sequences of mapped markers of grass carp against the whole genome sequence of zebrafish revealed substantial macrosynteny relationship and extensive colinearity of markers between grass carp and zebrafish.Conclusions: The linkage map of grass carp presented here is the first linkage map of a food fish species based on co-dominant markers in the family Cyprinidae. This map provides a valuable resource for mapping phenotypic variations and serves as a reference to approach comparative genomics and understand the evolution of fish genomes and could be complementary to grass carp genome sequencing project. © 2010 Xia et al; licensee BioMed Central Ltd. | Source Title: | BMC Genomics | URI: | https://scholarbank.nus.edu.sg/handle/10635/174458 | ISSN: | 14712164 | DOI: | 10.1186/1471-2164-11-135 |
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