Please use this identifier to cite or link to this item:
https://doi.org/10.1038/s41598-017-00438-8
Title: | Transcriptome and functional analysis reveals hybrid vigor for oil biosynthesis in oil palm | Authors: | Jin, J Sun, Y Qu, J Syah, R Lim, C.-H Alfiko, Y Rahman, N Suwanto, A Yue, G Wong, L Chua, N.-H Ye, J |
Keywords: | Arabidopsis protein lipid palm oil transcription factor WRINKLED1 protein, Arabidopsis Arabidopsis Arecaceae biosynthesis chimera deficiency gene expression profiling genetic complementation genetics heterosis metabolism Arabidopsis Arabidopsis Proteins Arecaceae Biosynthetic Pathways Chimera Gene Expression Profiling Genetic Complementation Test Hybrid Vigor Lipids Palm Oil Transcription Factors |
Issue Date: | 2017 | Publisher: | Nature Publishing Group | Citation: | Jin, J, Sun, Y, Qu, J, Syah, R, Lim, C.-H, Alfiko, Y, Rahman, N, Suwanto, A, Yue, G, Wong, L, Chua, N.-H, Ye, J (2017). Transcriptome and functional analysis reveals hybrid vigor for oil biosynthesis in oil palm. Scientific Reports 7 (1) : 439. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-017-00438-8 | Abstract: | Oil palm is the most productive oil crop in the world and composes 36% of the world production. However, the molecular mechanisms of hybrids vigor (or heterosis) between Dura, Pisifera and their hybrid progeny Tenera has not yet been well understood. Here we compared the temporal and spatial compositions of lipids and transcriptomes for two oil yielding organs mesocarp and endosperm from Dura, Pisifera and Tenera. Multiple lipid biosynthesis pathways are highly enriched in all non-additive expression pattern in endosperm, while cytokinine biosynthesis and cell cycle pathways are highly enriched both in endosperm and mesocarp. Compared with parental palms, the high oil content in Tenera was associated with much higher transcript levels of EgWRI1, homolog of Arabidopsis thaliana WRINKLED1. Among 338 identified genes in lipid synthesis, 207 (61%) has been identified to contain the WRI1 specific binding AW motif. We further functionally identified EgWRI1-1, one of three EgWRI1 orthologs, by genetic complementation of the Arabidopsis wri1 mutant. Ectopic expression of EgWRI1-1 in plant produced dramatically increased seed mass and oil content, with oil profile changed. Our findings provide an explanation for EgWRI1 as an important gene contributing hybrid vigor in lipid biosynthesis in oil palm. © 2017 The Author(s). | Source Title: | Scientific Reports | URI: | https://scholarbank.nus.edu.sg/handle/10635/174414 | ISSN: | 2045-2322 | DOI: | 10.1038/s41598-017-00438-8 |
Appears in Collections: | Elements Staff Publications |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_1038_s41598-017-00438-8.pdf | 2.83 MB | Adobe PDF | OPEN | None | View/Download |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.