Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41598-018-20305-4
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dc.titleAntibody-mediated enhancement aggravates chikungunya virus infection and disease severity
dc.contributor.authorLum, F.-M
dc.contributor.authorCouderc, T
dc.contributor.authorChia, B.-S
dc.contributor.authorOng, R.-Y
dc.contributor.authorHer, Z
dc.contributor.authorChow, A
dc.contributor.authorLeo, Y.-S
dc.contributor.authorKam, Y.-W
dc.contributor.authorRénia, L
dc.contributor.authorLecuit, M
dc.contributor.authorNg, L.F.P
dc.date.accessioned2020-09-04T02:19:36Z
dc.date.available2020-09-04T02:19:36Z
dc.date.issued2018
dc.identifier.citationLum, F.-M, Couderc, T, Chia, B.-S, Ong, R.-Y, Her, Z, Chow, A, Leo, Y.-S, Kam, Y.-W, Rénia, L, Lecuit, M, Ng, L.F.P (2018). Antibody-mediated enhancement aggravates chikungunya virus infection and disease severity. Scientific Reports 8 (1) : 1860. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-018-20305-4
dc.identifier.issn2045-2322
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/174342
dc.description.abstractThe arthropod-transmitted chikungunya virus (CHIKV) causes a flu-like disease that is characterized by incapacitating arthralgia. The re-emergence of CHIKV and the continual risk of new epidemics have reignited research in CHIKV pathogenesis. Virus-specific antibodies have been shown to control virus clearance, but antibodies present at sub-neutralizing concentrations can also augment virus infection that exacerbates disease severity. To explore this occurrence, CHIKV infection was investigated in the presence of CHIKV-specific antibodies in both primary human cells and a murine macrophage cell line, RAW264.7. Enhanced attachment of CHIKV to the primary human monocytes and B cells was observed while increased viral replication was detected in RAW264.7 cells. Blocking of specific Fc receptors (Fc?Rs) led to the abrogation of these observations. Furthermore, experimental infection in adult mice showed that animals had higher viral RNA loads and endured more severe joint inflammation in the presence of sub-neutralizing concentrations of CHIKV-specific antibodies. In addition, CHIKV infection in 11 days old mice under enhancing condition resulted in higher muscles viral RNA load detected and death. These observations provide the first evidence of antibody-mediated enhancement in CHIKV infection and pathogenesis and could also be relevant for other important arboviruses such as Zika virus. © 2018 The Author(s).
dc.publisherNature Publishing Group
dc.sourceUnpaywall 20200831
dc.subjectFc receptor
dc.subjectFc receptor IIa
dc.subjectgamma interferon
dc.subjectneutralizing antibody
dc.subjectvirus antibody
dc.subjectanimal
dc.subjectarthralgia
dc.subjectC57BL mouse
dc.subjectcell culture
dc.subjectchikungunya
dc.subjectChikungunya virus
dc.subjecthuman
dc.subjectimmunology
dc.subjectknockout mouse
dc.subjectmacrophage
dc.subjectmetabolism
dc.subjectmouse
dc.subjectpathology
dc.subjectphysiology
dc.subjectseverity of illness index
dc.subjectvirology
dc.subjectvirus load
dc.subjectvirus replication
dc.subjectAnimals
dc.subjectAntibodies, Neutralizing
dc.subjectAntibodies, Viral
dc.subjectArthralgia
dc.subjectCells, Cultured
dc.subjectChikungunya Fever
dc.subjectChikungunya virus
dc.subjectHumans
dc.subjectInterferon-gamma
dc.subjectMacrophages
dc.subjectMice
dc.subjectMice, Inbred C57BL
dc.subjectMice, Knockout
dc.subjectReceptors, IgG
dc.subjectSeverity of Illness Index
dc.subjectViral Load
dc.subjectVirus Replication
dc.typeArticle
dc.contributor.departmentSAW SWEE HOCK SCHOOL OF PUBLIC HEALTH
dc.contributor.departmentDEPT OF MICROBIOLOGY & IMMUNOLOGY
dc.contributor.departmentDEPT OF BIOCHEMISTRY
dc.description.doi10.1038/s41598-018-20305-4
dc.description.sourcetitleScientific Reports
dc.description.volume8
dc.description.issue1
dc.description.page1860
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