Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41467-018-03077-3
Title: Basal condensation of Numb and Pon complex via phase transition during Drosophila neuroblast asymmetric division
Authors: Shan, Z
Tu, Y
Yang, Y 
Liu, Z
Zeng, M
Xu, H
Long, J
Zhang, M
Cai, Y 
Wen, W
Keywords: Numb protein
Pon complex
protein
unclassified drug
carrier protein
Drosophila protein
juvenile hormone
numb protein, Drosophila
partner of Numb protein, Drosophila
protein binding
bioactivity
biochemistry
cell
chemical binding
condensation
fly
phase transition
physiological response
protein
separation
amino terminal sequence
Article
asymmetric cell division
controlled study
Drosophila
in vitro study
neuroblast
nonhuman
Notch signaling
phase separation
phase transition
protein binding
protein localization
protein motif
protein protein interaction
animal
cell differentiation
cell membrane
cell polarity
Drosophila melanogaster
embryology
gene expression regulation
HEK293 cell line
HeLa cell line
human
metabolism
nerve cell
nervous system development
physiology
protein domain
signal transduction
Amino Acid Motifs
Animals
Asymmetric Cell Division
Carrier Proteins
Cell Differentiation
Cell Membrane
Cell Polarity
Drosophila melanogaster
Drosophila Proteins
Gene Expression Regulation, Developmental
HEK293 Cells
HeLa Cells
Humans
Juvenile Hormones
Neurogenesis
Neurons
Protein Binding
Protein Domains
Signal Transduction
Issue Date: 2018
Publisher: Nature Publishing Group
Citation: Shan, Z, Tu, Y, Yang, Y, Liu, Z, Zeng, M, Xu, H, Long, J, Zhang, M, Cai, Y, Wen, W (2018). Basal condensation of Numb and Pon complex via phase transition during Drosophila neuroblast asymmetric division. Nature Communications 9 (1) : 737. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-018-03077-3
Abstract: Uneven distribution and local concentration of protein complexes on distinct membrane cortices is a fundamental property in numerous biological processes, including Drosophila neuroblast (NB) asymmetric cell divisions and cell polarity in general. In NBs, the cell fate determinant Numb forms a basal crescent together with Pon and is segregated into the basal daughter cell to initiate its differentiation. Here we discover that Numb PTB domain, using two distinct binding surfaces, recognizes repeating motifs within Pon in a previously unrecognized mode. The multivalent Numb-Pon interaction leads to high binding specificity and liquid-liquid phase separation of the complex. Perturbations of the Numb/Pon complex phase transition impair the basal localization of Numb and its subsequent suppression of Notch signaling during NB asymmetric divisions. Such phase-transition-mediated protein condensations on distinct membrane cortices may be a general mechanism for various cell polarity regulatory complexes. © 2018 The Author(s).
Source Title: Nature Communications
URI: https://scholarbank.nus.edu.sg/handle/10635/174325
ISSN: 2041-1723
DOI: 10.1038/s41467-018-03077-3
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