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https://doi.org/10.1038/s41467-018-03422-6
Title: | CoA synthase regulates mitotic fidelity via CBP-mediated acetylation | Authors: | Lin, C.-C Kitagawa, M Tang, X Hou, M.-H Wu, J Qu, D.C Srinivas, V Liu, X Thompson, J.W Mathey-Prevot, B Yao, T.-P Lee, S.H Chi, J.-T |
Keywords: | acyltransferase alpha tubulin aurora A kinase beta tubulin coenzyme A coenzyme A synthase cyclic AMP responsive element binding protein binding protein cyclin B1 cycloheximide enzyme messenger RNA protein top2a protein tpx2 protein unclassified drug aurora A kinase cell cycle protein COASY protein, human cyclic AMP responsive element binding protein binding protein microtubule associated protein nuclear protein protein binding TPX2 protein, human transferase chemical analysis chemical reaction enzyme enzyme activity molecular analysis protein acetylation Article cancer cell culture controlled study cytokinesis enzyme activation enzyme activity gene inactivation genomic instability human human cell in vitro study interphase mitosis protein degradation protein stability triple negative breast cancer ubiquitination acetylation cell line genetics metabolism Acetylation Aurora Kinase A Cell Cycle Proteins Cell Line CREB-Binding Protein Humans Microtubule-Associated Proteins Mitosis Nuclear Proteins Protein Binding Transferases |
Issue Date: | 2018 | Publisher: | Nature Publishing Group | Citation: | Lin, C.-C, Kitagawa, M, Tang, X, Hou, M.-H, Wu, J, Qu, D.C, Srinivas, V, Liu, X, Thompson, J.W, Mathey-Prevot, B, Yao, T.-P, Lee, S.H, Chi, J.-T (2018). CoA synthase regulates mitotic fidelity via CBP-mediated acetylation. Nature Communications 9 (1) : 1039. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-018-03422-6 | Abstract: | The temporal activation of kinases and timely ubiquitin-mediated degradation is central to faithful mitosis. Here we present evidence that acetylation controlled by Coenzyme A synthase (COASY) and acetyltransferase CBP constitutes a novel mechanism that ensures faithful mitosis. We found that COASY knockdown triggers prolonged mitosis and multinucleation. Acetylome analysis reveals that COASY inactivation leads to hyper-acetylation of proteins associated with mitosis, including CBP and an Aurora A kinase activator, TPX2. During early mitosis, a transient CBP-mediated TPX2 acetylation is associated with TPX2 accumulation and Aurora A activation. The recruitment of COASY inhibits CBP-mediated TPX2 acetylation, promoting TPX2 degradation for mitotic exit. Consistently, we detected a stage-specific COASY-CBP-TPX2 association during mitosis. Remarkably, pharmacological and genetic inactivation of CBP effectively rescued the mitotic defects caused by COASY knockdown. Together, our findings uncover a novel mitotic regulation wherein COASY and CBP coordinate an acetylation network to enforce productive mitosis. © 2018 The Author(s). | Source Title: | Nature Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/174236 | ISSN: | 2041-1723 | DOI: | 10.1038/s41467-018-03422-6 |
Appears in Collections: | Elements Staff Publications |
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