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https://doi.org/10.1038/s41467-018-03904-7
Title: | Global H3.3 dynamic deposition defines its bimodal role in cell fate transition | Authors: | Fang, H.-T El Farran, C.A Xing, Q.R Zhang, L.-F Li, H Lim, B Loh, Y.-H |
Keywords: | histone H3 kruppel like factor 4 mitochondrial DNA mitogen activated protein kinase Myc protein octamer transcription factor 4 transcription factor Otx2 transcription factor Sox2 chaperone collagen histone protein binding transcriptome cell collagen differentiation gene expression genetic analysis protein animal cell Article cell fate cell proliferation cell transdifferentiation collagen metabolism collagen synthesis controlled study embryo embryonic stem cell fibroblast mouse nerve cell differentiation nonhuman nuclear reprogramming nucleosome promoter region protein expression animal cell differentiation cell lineage chemistry chromatin immunoprecipitation cytology genetics HEK293 cell line heterochromatin human MAPK signaling metabolism nerve cell pluripotent stem cell Retroviridae software Animals Cell Differentiation Cell Lineage Chromatin Immunoprecipitation Collagen Fibroblasts HEK293 Cells Heterochromatin Histone Chaperones Histones Humans MAP Kinase Signaling System Mice Neurons Nucleosomes Pluripotent Stem Cells Protein Binding Retroviridae Software Transcriptome |
Issue Date: | 2018 | Publisher: | Nature Publishing Group | Citation: | Fang, H.-T, El Farran, C.A, Xing, Q.R, Zhang, L.-F, Li, H, Lim, B, Loh, Y.-H (2018). Global H3.3 dynamic deposition defines its bimodal role in cell fate transition. Nature Communications 9 (1) : 1537. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-018-03904-7 | Abstract: | H3.3 is a histone variant, which is deposited on genebodies and regulatory elements, by Hira, marking active transcription. Moreover, H3.3 is deposited on heterochromatin by Atrx/Daxx complex. The exact role of H3.3 in cell fate transition remains elusive. Here, we investigate the dynamic changes in the deposition of the histone variant H3.3 during cellular reprogramming. H3.3 maintains the identities of the parental cells during reprogramming as its removal at early time-point enhances the efficiency of the process. We find that H3.3 plays a similar role in transdifferentiation to hematopoietic progenitors and neuronal differentiation from embryonic stem cells. Contrastingly, H3.3 deposition on genes associated with the newly reprogrammed lineage is essential as its depletion at the later phase abolishes the process. Mechanistically, H3.3 deposition by Hira, and its K4 and K36 modifications are central to the role of H3.3 in cell fate conversion. Finally, H3.3 safeguards fibroblast lineage by regulating Mapk cascade and collagen synthesis. © 2018 The Author(s). | Source Title: | Nature Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/174227 | ISSN: | 2041-1723 | DOI: | 10.1038/s41467-018-03904-7 |
Appears in Collections: | Elements Staff Publications |
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