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https://doi.org/10.18632/oncotarget.8451
Title: | Capsules of virulent pneumococcal serotypes enhance formation of neutrophil extracellular traps during in vivo pathogenesis of pneumonia | Authors: | Moorthy, A.N Rai, P Jiao, H Wang, S Tan, K.B Qin, L Watanabe, H Zhang, Y Teluguakula, N Chow, V.T.K |
Keywords: | cytokine interleukin 10 interleukin 17 interleukin 1beta interleukin 6 myeloperoxidase tumor necrosis factor alpha cytokine animal cell animal experiment animal model animal tissue Article bacterial capsule bacterial strain bacterial virulence controlled study disease severity enzyme activity extracellular trap female in vivo study influenza innate immunity mouse nonhuman pathogenesis pneumonia serotype Streptococcus pneumoniae animal bacterial capsule Bagg albino mouse classification extracellular trap gene expression genetics host pathogen interaction immunology lung metabolism microbiology mutation neutrophil pathology serotyping Streptococcus pneumonia Streptococcus pneumoniae virulence Animals Bacterial Capsules Cytokines Extracellular Traps Female Gene Expression Host-Pathogen Interactions Lung Mice, Inbred BALB C Mutation Neutrophils Pneumonia, Pneumococcal Serotyping Streptococcus pneumoniae Virulence |
Issue Date: | 2016 | Citation: | Moorthy, A.N, Rai, P, Jiao, H, Wang, S, Tan, K.B, Qin, L, Watanabe, H, Zhang, Y, Teluguakula, N, Chow, V.T.K (2016). Capsules of virulent pneumococcal serotypes enhance formation of neutrophil extracellular traps during in vivo pathogenesis of pneumonia. Oncotarget 7 (15) : 19327-19340. ScholarBank@NUS Repository. https://doi.org/10.18632/oncotarget.8451 | Abstract: | Neutrophil extracellular traps (NETs) are released by activated neutrophils to ensnare and kill microorganisms. NETs have been implicated in tissue injury since they carry cytotoxic components of the activated neutrophils. We have previously demonstrated the generation of NETs in infected murine lungs during both primary pneumococcal pneumonia and secondary pneumococcal pneumonia after primary influenza. In this study, we assessed the correlation of pneumococcal capsule size with pulmonary NETs formation and disease severity. We compared NETs formation in the lungs of mice infected with three pneumococcal strains of varying virulence namely serotypes 3, 4 and 19F, as well as a capsule-deficient mutant of serotype 4. In primary pneumonia, NETs generation was strongly associated with the pneumococcal capsule thickness, and was proportional to the disease severity. Interestingly, during secondary pneumonia after primary influenza infection, intense pulmonary NETs generation together with elevated myeloperoxidase activity and cytokine dysregulation determined the disease severity. These findings highlight the crucial role played by the size of pneumococcal capsule in determining the extent of innate immune responses such as NETs formation that may contribute to the severity of pneumonia. | Source Title: | Oncotarget | URI: | https://scholarbank.nus.edu.sg/handle/10635/174096 | ISSN: | 19492553 | DOI: | 10.18632/oncotarget.8451 |
Appears in Collections: | Elements Staff Publications |
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