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https://doi.org/10.18632/oncotarget.8875
Title: | ANXA1 inhibits miRNA-196a in a negative feedback loop through NF-kB and C-Myc to reduce breast cancer proliferation | Authors: | Yuan, Y Anbalagan, D Lee, L.H Samy, R.P Shanmugam, M.K Kumar, A.P Sethi, G Lobie, P.E Lim, L.H.K |
Keywords: | annexin annexin 1 estrogen receptor immunoglobulin enhancer binding protein microRNA microRNA 196a Myc protein synaptotagmin I unclassified drug immunoglobulin enhancer binding protein lipocortin 1 microRNA MIRN196 microRNA, human Myc protein MYC protein, human Article breast cancer cancer inhibition cell proliferation controlled study gene expression human human cell in vivo study MCF 7 cell line protein function RNA transcription tumor growth animal Bagg albino mouse breast tumor cell proliferation female gene expression regulation genetics MCF-7 cell line metabolism nude mouse pathology physiological feedback RNA interference tumor cell line tumor volume xenograft Animals Annexin A1 Breast Neoplasms Cell Line, Tumor Cell Proliferation Feedback, Physiological Female Gene Expression Regulation, Neoplastic Humans MCF-7 Cells Mice, Inbred BALB C Mice, Nude MicroRNAs NF-kappa B Proto-Oncogene Proteins c-myc RNA Interference Transplantation, Heterologous Tumor Burden |
Issue Date: | 2016 | Citation: | Yuan, Y, Anbalagan, D, Lee, L.H, Samy, R.P, Shanmugam, M.K, Kumar, A.P, Sethi, G, Lobie, P.E, Lim, L.H.K (2016). ANXA1 inhibits miRNA-196a in a negative feedback loop through NF-kB and C-Myc to reduce breast cancer proliferation. Oncotarget 7 (19) : 27007-27020. ScholarBank@NUS Repository. https://doi.org/10.18632/oncotarget.8875 | Abstract: | MiRNAs are endogenous ~22 nt RNAs which play critical regulatory roles in a wide range of biological and pathological processes, which can act as oncogenes or tumor suppressor genes depending on their target genes. We have recently shown that ANXA1 inhibits the expression of miRNAs including miR196a. Here, we show that miR196a was highly expressed in ER+ MCF-7 breast cancer cells when compared to normal mammary gland cells, with expression levels negatively correlating to ANXA1. ANXA1 inhibits the biogenesis of oncogenic miR-196a by suppressing primarymiR196a indirectly through the stimulation of c-myc and NFkB expression and activity in breast cancer cells. In a negative feedback loop, miR-196a directly inhibits ANXA1 and enhances breast cancer cell proliferation in vitro. Finally, miR196a promotes breast tumor growth in vivo. This study reports a novel regulatory circuit between ANXA1, NF-kB, c-myc and miR-196a which regulates breast cancer cell proliferation and tumor growth. | Source Title: | Oncotarget | URI: | https://scholarbank.nus.edu.sg/handle/10635/174092 | ISSN: | 19492553 | DOI: | 10.18632/oncotarget.8875 |
Appears in Collections: | Elements Staff Publications |
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