Please use this identifier to cite or link to this item: https://doi.org/10.3390/pathogens5010009
Title: Application and optimization of relE as a negative selection marker for making definitive genetic constructs in uropathogenic escherichia coli
Authors: Khetrapal, V 
Mehershahi, K.S 
Chen, S 
Chen, S.L 
Keywords: virulence factor
Article
bacterial strain
gene construct
gene mutation
genetic organization
hemagglutination
nonhuman
phenotype
plasmid
polymerase chain reaction
sequence analysis
uropathogenic Escherichia coli
Issue Date: 2016
Citation: Khetrapal, V, Mehershahi, K.S, Chen, S, Chen, S.L (2016). Application and optimization of relE as a negative selection marker for making definitive genetic constructs in uropathogenic escherichia coli. Pathogens 5 (1) : 1-6. ScholarBank@NUS Repository. https://doi.org/10.3390/pathogens5010009
Abstract: Studies of Uropathogenic Escherichia coli (UPEC) pathogenesis have relied heavily on genetic manipulation to understand virulence factors. We applied a recently reported positive-negative selection system to create a series of unmarked, scarless FimH mutants that show identical phenotypes to previously reported marked FimH mutants; these are now improved versions useful for definitive assignment of phenotypes to FimH mutations. We also increased the efficiency of this system by designing new primer sites, which should further improve the efficiency and convenience of using negative selection in UTI89, other UPEC, and other Enterobacteriaceae. © 2016 by the authors; licensee MDPI, Basel, Switzerland.
Source Title: Pathogens
URI: https://scholarbank.nus.edu.sg/handle/10635/174032
ISSN: 20760817
DOI: 10.3390/pathogens5010009
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