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https://doi.org/10.1038/srep19672
Title: | Topography induces differential sensitivity on cancer cell proliferation via Rho-ROCK-Myosin contractility | Authors: | Chaudhuri, P.K Pan, C.Q Low, B.C Lim, C.T |
Keywords: | collagen myosin Rho guanine nucleotide binding protein Rho kinase animal bovine breast tumor cell motion cell proliferation chemistry drug effects epithelium cell extracellular matrix female human metabolism metastasis pathology signal transduction tumor cell line Animals Breast Neoplasms Cattle Cell Line, Tumor Cell Movement Cell Proliferation Collagen Epithelial Cells Extracellular Matrix Female Humans Myosins Neoplasm Metastasis rho GTP-Binding Proteins rho-Associated Kinases Signal Transduction |
Issue Date: | 2016 | Citation: | Chaudhuri, P.K, Pan, C.Q, Low, B.C, Lim, C.T (2016). Topography induces differential sensitivity on cancer cell proliferation via Rho-ROCK-Myosin contractility. Scientific Reports 6 : 19672. ScholarBank@NUS Repository. https://doi.org/10.1038/srep19672 | Abstract: | Although the role of stiffness on proliferative response of cancer cells has been well studied, little is known about the effect of topographic cues in guiding cancer cell proliferation. Here, we examined the effect of topographic cues on cancer cell proliferation using micron scale topographic features and observed that anisotropic features like microgratings at specific dimension could reduce proliferation of non-cancer breast epithelial cells (MCF-10A) but not that for malignant breast cancer cells (MDA-MB-231 and MCF-7). However, isotropic features such as micropillars did not affect proliferation of MCF-10A, indicating that the anisotropic environmental cues are essential for this process. Interestingly, acto-myosin contraction inhibitory drugs, Y-27632 and blebbistatin prevented micrograting-mediated inhibition on proliferation. Here, we propose the concept of Mechanically-Induced Dormancy (MID) where topographic cues could activate Rho-ROCK-Myosin signaling to suppress non-cancerous cells proliferation whereas malignant cells are resistant to this inhibitory barrier and therefore continue uncontrolled proliferation. | Source Title: | Scientific Reports | URI: | https://scholarbank.nus.edu.sg/handle/10635/174030 | ISSN: | 20452322 | DOI: | 10.1038/srep19672 |
Appears in Collections: | Elements Staff Publications |
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