Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep19672
Title: Topography induces differential sensitivity on cancer cell proliferation via Rho-ROCK-Myosin contractility
Authors: Chaudhuri, P.K
Pan, C.Q 
Low, B.C 
Lim, C.T 
Keywords: collagen
myosin
Rho guanine nucleotide binding protein
Rho kinase
animal
bovine
breast tumor
cell motion
cell proliferation
chemistry
drug effects
epithelium cell
extracellular matrix
female
human
metabolism
metastasis
pathology
signal transduction
tumor cell line
Animals
Breast Neoplasms
Cattle
Cell Line, Tumor
Cell Movement
Cell Proliferation
Collagen
Epithelial Cells
Extracellular Matrix
Female
Humans
Myosins
Neoplasm Metastasis
rho GTP-Binding Proteins
rho-Associated Kinases
Signal Transduction
Issue Date: 2016
Citation: Chaudhuri, P.K, Pan, C.Q, Low, B.C, Lim, C.T (2016). Topography induces differential sensitivity on cancer cell proliferation via Rho-ROCK-Myosin contractility. Scientific Reports 6 : 19672. ScholarBank@NUS Repository. https://doi.org/10.1038/srep19672
Abstract: Although the role of stiffness on proliferative response of cancer cells has been well studied, little is known about the effect of topographic cues in guiding cancer cell proliferation. Here, we examined the effect of topographic cues on cancer cell proliferation using micron scale topographic features and observed that anisotropic features like microgratings at specific dimension could reduce proliferation of non-cancer breast epithelial cells (MCF-10A) but not that for malignant breast cancer cells (MDA-MB-231 and MCF-7). However, isotropic features such as micropillars did not affect proliferation of MCF-10A, indicating that the anisotropic environmental cues are essential for this process. Interestingly, acto-myosin contraction inhibitory drugs, Y-27632 and blebbistatin prevented micrograting-mediated inhibition on proliferation. Here, we propose the concept of Mechanically-Induced Dormancy (MID) where topographic cues could activate Rho-ROCK-Myosin signaling to suppress non-cancerous cells proliferation whereas malignant cells are resistant to this inhibitory barrier and therefore continue uncontrolled proliferation.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/174030
ISSN: 20452322
DOI: 10.1038/srep19672
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