Please use this identifier to cite or link to this item: https://doi.org/10.7554/eLife.10747
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dc.titleThe horizontally-acquired response regulator SsrB drives a Salmonella lifestyle switch by relieving biofilm silencing
dc.contributor.authorDesai S.K.
dc.contributor.authorWinardhi R.S.
dc.contributor.authorPeriasamy S.
dc.contributor.authorDykas M.M.
dc.contributor.authorJie Y.
dc.contributor.authorKenney L.J.
dc.date.accessioned2020-09-02T06:59:56Z
dc.date.available2020-09-02T06:59:56Z
dc.date.issued2016
dc.identifier.citationDesai S.K., Winardhi R.S., Periasamy S., Dykas M.M., Jie Y., Kenney L.J. (2016). The horizontally-acquired response regulator SsrB drives a Salmonella lifestyle switch by relieving biofilm silencing. eLife 5 (42401) : e10747. ScholarBank@NUS Repository. https://doi.org/10.7554/eLife.10747
dc.identifier.issn2050084X
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/174028
dc.description.abstractA common strategy by which bacterial pathogens reside in humans is by shifting from a virulent lifestyle, (systemic infection), to a dormant carrier state. Two major serovars of Salmonella enterica, Typhi and Typhimurium, have evolved a two-component regulatory system to exist inside Salmonella-containing vacuoles in the macrophage, as well as to persist as asymptomatic biofilms in the gallbladder. Here we present evidence that SsrB, a transcriptional regulator encoded on the SPI-2 pathogenicity-island, determines the switch between these two lifestyles by controlling ancestral and horizontally-acquired genes. In the acidic macrophage vacuole, the kinase SsrA phosphorylates SsrB, and SsrB~P relieves silencing of virulence genes and activates their transcription. In the absence of SsrA, unphosphorylated SsrB directs transcription of factors required for biofilm formation specifically by activating csgD (agfD), the master biofilm regulator by disrupting the silenced, H-NS-bound promoter. Anti-silencing mechanisms thus control the switch between opposing lifestyles. © Desai et al.
dc.sourceUnpaywall 20200831
dc.subjectArticle
dc.subjectatomic force microscopy
dc.subjectbacterial growth
dc.subjectbacterial strain
dc.subjectbiofilm
dc.subjectdown regulation
dc.subjectfluorescence microscopy
dc.subjectgene
dc.subjectgene silencing
dc.subjectimmunoblotting
dc.subjectmolecular biology
dc.subjectnonhuman
dc.subjectprotein expression
dc.subjectprotein phosphorylation
dc.subjectprotein purification
dc.subjectreal time polymerase chain reaction
dc.subjectreverse transcription polymerase chain reaction
dc.subjectSalmonella
dc.subjectSalmonella enterica serovar Typhi
dc.subjectSalmonella enterica serovar Typhimurium
dc.subjectscanning electron microscopy
dc.subjectssrB gene
dc.subjectvirulence
dc.subjectWestern blotting
dc.subjectbiofilm
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectgenomic island
dc.subjectgrowth, development and aging
dc.subjectmetabolism
dc.subjectphysiology
dc.subjectbacterial protein
dc.subjectSsrB protein, Salmonella typhimurium
dc.subjecttranscription factor
dc.subjectBacterial Proteins
dc.subjectBiofilms
dc.subjectGene Expression Regulation, Bacterial
dc.subjectGenomic Islands
dc.subjectSalmonella typhimurium
dc.subjectTranscription Factors
dc.typeArticle
dc.contributor.departmentMECHANOBIOLOGY INSTITUTE
dc.contributor.departmentNUS NANOSCIENCE & NANOTECH INITIATIVE
dc.description.doi10.7554/eLife.10747
dc.description.sourcetitleeLife
dc.description.volume5
dc.description.issue42401
dc.description.pagee10747
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