Please use this identifier to cite or link to this item:
https://doi.org/10.1038/srep19943
DC Field | Value | |
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dc.title | GRHL2-miR-200-ZEB1 maintains the epithelial status of ovarian cancer through transcriptional regulation and histone modification | |
dc.contributor.author | Chung, V.Y | |
dc.contributor.author | Tan, T.Z | |
dc.contributor.author | Tan, M | |
dc.contributor.author | Wong, M.K | |
dc.contributor.author | Kuay, K.T | |
dc.contributor.author | Yang, Z | |
dc.contributor.author | Ye, J | |
dc.contributor.author | Muller, J | |
dc.contributor.author | Koh, C.M | |
dc.contributor.author | Guccione, E | |
dc.contributor.author | Thiery, J.P | |
dc.contributor.author | Huang, R.Y.-J | |
dc.date.accessioned | 2020-09-02T06:58:26Z | |
dc.date.available | 2020-09-02T06:58:26Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Chung, V.Y, Tan, T.Z, Tan, M, Wong, M.K, Kuay, K.T, Yang, Z, Ye, J, Muller, J, Koh, C.M, Guccione, E, Thiery, J.P, Huang, R.Y.-J (2016). GRHL2-miR-200-ZEB1 maintains the epithelial status of ovarian cancer through transcriptional regulation and histone modification. Scientific Reports 6 : 19943. ScholarBank@NUS Repository. https://doi.org/10.1038/srep19943 | |
dc.identifier.issn | 20452322 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/174023 | |
dc.description.abstract | Epithelial-mesenchymal transition (EMT), a biological process by which polarized epithelial cells convert into a mesenchymal phenotype, has been implicated to contribute to the molecular heterogeneity of epithelial ovarian cancer (EOC). Here we report that a transcription factor-Grainyhead-like 2 (GRHL2) maintains the epithelial phenotype. EOC tumours with lower GRHL2 levels are associated with the Mes/Mesenchymal molecular subtype and a poorer overall survival. shRNA-mediated knockdown of GRHL2 in EOC cells with an epithelial phenotype results in EMT changes, with increased cell migration, invasion and motility. By ChIP-sequencing and gene expression microarray, microRNA-200b/a is identified as the direct transcriptional target of GRHL2 and regulates the epithelial status of EOC through ZEB1 and E-cadherin. Our study demonstrates that loss of GRHL2 increases the levels of histone mark H3K27me3 on promoters and GRHL2-binding sites at miR-200b/a and E-cadherin genes. These findings support GRHL2 as a pivotal gatekeeper of EMT in EOC via miR-200-ZEB1. | |
dc.source | Unpaywall 20200831 | |
dc.subject | DNA binding protein | |
dc.subject | GRHL2 protein, human | |
dc.subject | histone | |
dc.subject | microRNA | |
dc.subject | MIRN200 microRNA, human | |
dc.subject | RNA | |
dc.subject | transcription factor | |
dc.subject | transcription factor ZEB1 | |
dc.subject | tumor protein | |
dc.subject | ZEB1 protein, human | |
dc.subject | DNA responsive element | |
dc.subject | epithelial mesenchymal transition | |
dc.subject | female | |
dc.subject | genetic transcription | |
dc.subject | genetics | |
dc.subject | human | |
dc.subject | metabolism | |
dc.subject | ovary tumor | |
dc.subject | pathology | |
dc.subject | protein processing | |
dc.subject | tumor cell line | |
dc.subject | Cell Line, Tumor | |
dc.subject | DNA-Binding Proteins | |
dc.subject | Epithelial-Mesenchymal Transition | |
dc.subject | Female | |
dc.subject | Histones | |
dc.subject | Humans | |
dc.subject | MicroRNAs | |
dc.subject | Neoplasm Proteins | |
dc.subject | Ovarian Neoplasms | |
dc.subject | Protein Processing, Post-Translational | |
dc.subject | Response Elements | |
dc.subject | RNA, Neoplasm | |
dc.subject | Transcription Factors | |
dc.subject | Transcription, Genetic | |
dc.subject | Zinc Finger E-box-Binding Homeobox 1 | |
dc.type | Article | |
dc.contributor.department | CANCER SCIENCE INSTITUTE OF SINGAPORE | |
dc.contributor.department | DEPT OF BIOCHEMISTRY | |
dc.description.doi | 10.1038/srep19943 | |
dc.description.sourcetitle | Scientific Reports | |
dc.description.volume | 6 | |
dc.description.page | 19943 | |
Appears in Collections: | Elements Staff Publications |
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