Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep29658
Title: Germline replacement by blastula cell transplantation in the fish medaka
Authors: Li M.
Hong N. 
Xu H.
Song J. 
Hong Y. 
Keywords: biodiversity
blastula
cell transplantation
embryo
fertility
gene overexpression
genetic model
germ line
human
human tissue
Oryzias latipes
primordial germ cell
animal
blastula
cell transplantation
chimera
germ cell
gonad
Oryzias
physiology
procedures
Animals
Biodiversity
Blastula
Cell Transplantation
Chimera
Germ Cells
Gonads
Oryzias
Issue Date: 2016
Citation: Li M., Hong N., Xu H., Song J., Hong Y. (2016). Germline replacement by blastula cell transplantation in the fish medaka. Scientific Reports 6 : 29658. ScholarBank@NUS Repository. https://doi.org/10.1038/srep29658
Abstract: Primordial germ cell (PGC) specification early in development establishes the germline for reproduction and reproductive technologies. Germline replacement (GR) is a powerful tool for conservation of valuable or endangered animals. GR is achievable by germ cell transplantation into the PGC migration pathway or gonads. Blastula cell transplantation (BCT) can also lead to the chimeric germline containing PGCs of both donor and host origins. It has remained largely unknown whether BCT is able to achieve GR at a high efficiency. Here we report efficient GR by BCT into blastula embryos in the fish medaka (Oryzias latipes). Specifically, dnd depletion completely ablated host PGCs and fertility, and dnd overexpression remarkably boosted PGCs in donor blastulae. BCT between normal donor and host produced a germline transmission rate of ?4%. This rate was enhanced up to ?30% upon PGC boosting in donors. Most importantly, BCT between PGC-boosted donors and PGC-ablated hosts led to more than 90% fertility restoration and 100% GR. Therefore, BCT features an extremely high efficiency of fertility recovery and GR in medaka. This finding makes medaka an ideal model to analyze genetic and physiological donor-host compatibilities for BCT-mediated surrogate production and propagation of endangered lower vertebrates and biodiversity.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/174002
ISSN: 20452322
DOI: 10.1038/srep29658
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