Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep29712
Title: Transcriptional and functional characterization of CD137L-dendritic cells identifies a novel dendritic cell phenotype
Authors: Harfuddin, Z 
Dharmadhikari, B 
Wong, S.C 
Duan, K
Poidinger, M 
Kwajah, S 
Schwarz, H 
Keywords: activated leukocyte cell adhesion molecule
CD137 ligand
cytokine
granulocyte macrophage colony stimulating factor
interleukin 4
cell differentiation
cell proliferation
dendritic cell
drug effect
gene expression profiling
genetics
human
metabolism
monocyte
phenotype
procedures
secretion (process)
signal transduction
transcription initiation
4-1BB Ligand
Activated-Leukocyte Cell Adhesion Molecule
Cell Differentiation
Cell Proliferation
Cytokines
Dendritic Cells
Gene Expression Profiling
Granulocyte-Macrophage Colony-Stimulating Factor
Humans
Interleukin-4
Monocytes
Phenotype
Signal Transduction
Transcriptional Activation
Issue Date: 2016
Citation: Harfuddin, Z, Dharmadhikari, B, Wong, S.C, Duan, K, Poidinger, M, Kwajah, S, Schwarz, H (2016). Transcriptional and functional characterization of CD137L-dendritic cells identifies a novel dendritic cell phenotype. Scientific Reports 6 : 29712. ScholarBank@NUS Repository. https://doi.org/10.1038/srep29712
Abstract: The importance of monocyte-derived dendritic cells (DCs) is evidenced by the fact that they are essential for the elimination of pathogens. Although in vitro DCs can be generated by treatment of monocytes with GM-CSF and IL-4, it is unknown what stimuli induce differentiation of DCs in vivo. CD137L-DCs are human monocyte-derived DC that are generated by CD137 ligand (CD137L) signaling. We demonstrate that the gene signature of in vitro generated CD137L-DCs is most similar to those of GM-CSF and IL-4-generated immature DCs and of macrophages. This is reminiscent of in vivo inflammatory DC which also have been reported to share gene signatures with monocyte-derived DCs and macrophages. Performing direct comparison of deposited human gene expression data with a CD137L-DC dataset revealed a significant enrichment of CD137L-DC signature genes in inflammatory in vivo DCs. In addition, surface marker expression and cytokine secretion by CD137L-DCs resemble closely those of inflammatory DCs. Further, CD137L-DCs express high levels of adhesion molecules, display strong attachment, and employ the adhesion molecule ALCAM to stimulate T cell proliferation. This study characterizes the gene expression profile of CD137L-DCs, and identifies significant similarities of CD137L-DCs with in vivo inflammatory monocyte-derived DCs and macrophages.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/174001
ISSN: 20452322
DOI: 10.1038/srep29712
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