Please use this identifier to cite or link to this item:
https://doi.org/10.7554/eLife.20770
DC Field | Value | |
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dc.title | Thalamo-cortical axons regulate the radial dispersion of neocortical GABAergic interneurons | |
dc.contributor.author | Zechel, S | |
dc.contributor.author | Nakagawa, Y | |
dc.contributor.author | Ibáñez, C.F | |
dc.date.accessioned | 2020-09-02T06:45:24Z | |
dc.date.available | 2020-09-02T06:45:24Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Zechel, S, Nakagawa, Y, Ibáñez, C.F (2016). Thalamo-cortical axons regulate the radial dispersion of neocortical GABAergic interneurons. eLife 5 (42705) : e20770. ScholarBank@NUS Repository. https://doi.org/10.7554/eLife.20770 | |
dc.identifier.issn | 2050084X | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/173976 | |
dc.description.abstract | Neocortical GABAergic interneuron migration and thalamo-cortical axon (TCA) pathfinding follow similar trajectories and timing, suggesting they may be interdependent. The mechanisms that regulate the radial dispersion of neocortical interneurons are incompletely understood. Here we report that disruption of TCA innervation, or TCA-derived glutamate, affected the laminar distribution of GABAergic interneurons in mouse neocortex, resulting in abnormal accumulation in deep layers of interneurons that failed to switch from tangential to radial orientation. Expression of the KCC2 cotransporter was elevated in interneurons of denervated cortex, and KCC2 deletion restored normal interneuron lamination in the absence of TCAs. Disruption of interneuron NMDA receptors or pharmacological inhibition of calpain also led to increased KCC2 expression and defective radial dispersion of interneurons. Thus, although TCAs are not required to guide the tangential migration of GABAergic interneurons, they provide crucial signals that restrict interneuron KCC2 levels, allowing coordinated neocortical invasion of TCAs and interneurons. © Zechel et al. | |
dc.source | Unpaywall 20200831 | |
dc.subject | 4 aminobutyric acid receptor | |
dc.subject | calpain | |
dc.subject | calpastatin | |
dc.subject | glutamic acid | |
dc.subject | n methyl dextro aspartic acid receptor | |
dc.subject | potassium chloride cotransporter 2 | |
dc.subject | tamoxifen | |
dc.subject | 4 aminobutyric acid receptor | |
dc.subject | animal tissue | |
dc.subject | Article | |
dc.subject | axon | |
dc.subject | brain cortex layer | |
dc.subject | cell migration | |
dc.subject | confocal microscopy | |
dc.subject | controlled study | |
dc.subject | denervation | |
dc.subject | electroporation | |
dc.subject | embryo | |
dc.subject | female | |
dc.subject | GABAergic system | |
dc.subject | image analysis | |
dc.subject | immunofluorescence test | |
dc.subject | immunohistochemistry | |
dc.subject | in situ hybridization | |
dc.subject | interneuron | |
dc.subject | mouse | |
dc.subject | neocortex | |
dc.subject | newborn | |
dc.subject | nonhuman | |
dc.subject | orientation | |
dc.subject | polymerase chain reaction | |
dc.subject | protein expression | |
dc.subject | radial dispersion | |
dc.subject | RNA probe | |
dc.subject | tangential orientation | |
dc.subject | thalamocortical tract | |
dc.subject | upregulation | |
dc.subject | animal | |
dc.subject | axon | |
dc.subject | brain cortex | |
dc.subject | cell motion | |
dc.subject | embryology | |
dc.subject | interneuron | |
dc.subject | nerve tract | |
dc.subject | physiology | |
dc.subject | thalamus | |
dc.subject | Animals | |
dc.subject | Axons | |
dc.subject | Cell Movement | |
dc.subject | Cerebral Cortex | |
dc.subject | GABAergic Neurons | |
dc.subject | Interneurons | |
dc.subject | Mice | |
dc.subject | Neural Pathways | |
dc.subject | Thalamus | |
dc.type | Article | |
dc.contributor.department | PHYSIOLOGY | |
dc.description.doi | 10.7554/eLife.20770 | |
dc.description.sourcetitle | eLife | |
dc.description.volume | 5 | |
dc.description.issue | 42705 | |
dc.description.page | e20770 | |
Appears in Collections: | Elements Staff Publications |
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10_7554_eLife_20770.pdf | 13.27 MB | Adobe PDF | OPEN | None | View/Download |
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