Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep46440
Title: LPS independent activation of the pro-inflammatory receptor Trem1 by C/EBP? in granulocytes
Authors: Suh, H.C
Benoukraf, T 
Shyamsunder, P 
Yin, T
Cao, Q
Said, J
Lee, S
Lim, R 
Yang, H 
Salotti, J
Johnson, P.F
Madan, V 
Koeffler, H.P 
Keywords: CCAAT enhancer binding protein
lipopolysaccharide
transcriptome
TREM1 protein, mouse
triggering receptor expressed on myeloid cells 1
animal
bone marrow cell
cell differentiation
granulocyte
metabolism
mouse
neutrophil
physiology
Animals
Bone Marrow Cells
CCAAT-Enhancer-Binding Proteins
Cell Differentiation
Granulocytes
Lipopolysaccharides
Mice
Neutrophils
Transcriptome
Triggering Receptor Expressed on Myeloid Cells-1
Issue Date: 2017
Citation: Suh, H.C, Benoukraf, T, Shyamsunder, P, Yin, T, Cao, Q, Said, J, Lee, S, Lim, R, Yang, H, Salotti, J, Johnson, P.F, Madan, V, Koeffler, H.P (2017). LPS independent activation of the pro-inflammatory receptor Trem1 by C/EBP? in granulocytes. Scientific Reports 7 : 46440. ScholarBank@NUS Repository. https://doi.org/10.1038/srep46440
Abstract: C/EBP? is a critical transcriptional factor for granulocyte differentiation and function. Individuals with germline mutations of C/EBP? fail to develop normal granulocytes and suffer from repeated infections. In order to gain a global view of the transcriptional machinery regulated by C/EBP?, we performed whole-genome ChIP-Seq using mouse bone marrow cells. To complement the C/EBP? DNA binding analyses, RNA-Sequencing was done in parallel using sorted mature and immature granulocytes from WT and C/EBP? KO bone marrow. This approach led to the identification of several direct targets of C/EBP?, which are potential effectors of its role in granulocytic differentiation and function. Interestingly, Trem1, a gene critical to granulocyte function, was identifed as a direct C/EBP? target gene. Trem1 expression overlaps very closely with expression signature of C/EBP? during hematopoietic development. Luciferase reporter and EMSA assays revealed that C/EBP? binds to the regulatory elements of Trem1 and regulates its expression during granulocytic differentiation. In addition, we provide evidence that inflammatory stimuli (LPS) can also control the expression of Trem1 independent of C/EBP?. Overall, this study provides comprehensive profiling of the transcriptional network controlled by C/EBP? during granulopoiesis and identifies Trem1 as one of its downstream effectors involved in eliciting an immune response. © The Author(s) 2017.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/173953
ISSN: 20452322
DOI: 10.1038/srep46440
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