Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep42816
Title: 25-Hydroxyvitamin D3 induces osteogenic differentiation of human mesenchymal stem cells
Authors: Lou, Y.-R
Toh, T.C 
Tee, Y.H 
Yu, H 
Keywords: alkaline phosphatase
calcifediol
colecalciferol 24 hydroxylase
CYP24A1 protein, human
dihydroxy-vitamin D3
osteocalcin
osteopontin
SPP1 protein, human
vitamin
vitamin D
analogs and derivatives
bone development
cell culture
cell differentiation
cytology
drug effect
gene expression regulation
genetics
human
mesenchymal stroma cell
metabolism
time factor
upregulation
Alkaline Phosphatase
Calcifediol
Cell Differentiation
Cells, Cultured
Gene Expression Regulation
Humans
Mesenchymal Stromal Cells
Osteocalcin
Osteogenesis
Osteopontin
Time Factors
Up-Regulation
Vitamin D
Vitamin D3 24-Hydroxylase
Vitamins
Issue Date: 2017
Citation: Lou, Y.-R, Toh, T.C, Tee, Y.H, Yu, H (2017). 25-Hydroxyvitamin D3 induces osteogenic differentiation of human mesenchymal stem cells. Scientific Reports 7 : 42816. ScholarBank@NUS Repository. https://doi.org/10.1038/srep42816
Abstract: 25-Hydroxyvitamin D3[25(OH)D3] has recently been found to be an active hormone. Its biological actions are demonstrated in various cell types. 25(OH)D3deficiency results in failure in bone formation and skeletal deformation. Here, we investigated the effect of 25(OH)D3on osteogenic differentiation of human mesenchymal stem cells (hMSCs). We also studied the effect of 1?,25-dihydroxyvitamin D3[1?,25-(OH) 2 D3], a metabolite of 25(OH)D3. One of the vitamin D responsive genes, 25(OH)D3-24-hydroxylase (cytochrome P450 family 24 subfamily A member 1) mRNA expression is up-regulated by 25(OH)D3at 250-500 nM and by 1?,25-(OH)2 D3at 1-10 nM. 25(OH)D3and 1?,25-(OH)2 D3at a time-dependent manner alter cell morphology towards osteoblast-associated characteristics. The osteogenic markers, alkaline phosphatase, secreted phosphoprotein 1 (osteopontin), and bone gamma-carboxyglutamate protein (osteocalcin) are increased by 25(OH)D3and 1?,25-OH)2 D3in a dose-dependent manner. Finally, mineralisation is significantly increased by 25(OH)D3but not by 1?,25-(OH)2 D3. Moreover, we found that hMSCs express very low level of 25(OH)D3-1?-hydroxylase (cytochrome P450 family 27 subfamily B member 1), and there is no detectable 1?,25-OH)2 D3product. Taken together, our findings provide evidence that 25(OH)D3at 250-500 nM can induce osteogenic differentiation and that 25(OH)D3has great potential for cell-based bone tissue engineering. © 2017 The Author(s).
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/173941
ISSN: 20452322
DOI: 10.1038/srep42816
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