Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep42963
Title: Detection of viral respiratory pathogens in mild and severe acute respiratory infections in Singapore
Authors: Jiang L. 
Lee V.J.M. 
Cui L.
Lin R. 
Tan C.L.
Tan L.W.L. 
Lim W.-Y. 
Leo Y.-S.
Low L.
Hibberd M.
Chen M.I.-C. 
Keywords: diagnosis
hospital patient
human
Influenza virus
major clinical study
nonhuman
polymerase chain reaction
respiratory virus
Singapore
adult
aged
Coronavirinae
female
genetics
Influenza A virus
isolation and purification
male
metabolism
middle aged
multiplex polymerase chain reaction
nasopharynx
pathology
prospective study
real time polymerase chain reaction
respiratory tract infection
Rhinovirus
severity of illness index
Singapore
virology
virus RNA
Adult
Aged
Coronavirus
Female
Humans
Influenza A virus
Male
Middle Aged
Multiplex Polymerase Chain Reaction
Nasopharynx
Prospective Studies
Real-Time Polymerase Chain Reaction
Respiratory Tract Infections
Rhinovirus
RNA, Viral
Severity of Illness Index
Singapore
Issue Date: 2017
Citation: Jiang L., Lee V.J.M., Cui L., Lin R., Tan C.L., Tan L.W.L., Lim W.-Y., Leo Y.-S., Low L., Hibberd M., Chen M.I.-C. (2017). Detection of viral respiratory pathogens in mild and severe acute respiratory infections in Singapore. Scientific Reports 7 : 42963. ScholarBank@NUS Repository. https://doi.org/10.1038/srep42963
Abstract: To investigate the performance of laboratory methods and clinical case definitions in detecting the viral pathogens for acute respiratory infections (ARIs) from a prospective community cohort and hospital inpatients, nasopharyngeal swabs from cohort members reporting ARIs (community-ARI) and inpatients admitted with ARIs (inpatient-ARI) were tested by Singleplex Real Time-Polymerase Chain Reaction (SRT-PCR), multiplex RT-PCR (MRT-PCR) and pathogen-chip system (PathChip) between April 2012 and December 2013. Community-ARI and inpatient-ARI was also combined with mild and severe cases of influenza from a historical prospective study as mild-ARI and severe-ARI respectively to evaluate the performance of clinical case definitions. We analysed 130 community-ARI and 140 inpatient-ARI episodes (5 inpatient-ARI excluded because multiple pathogens were detected), involving 138 and 207 samples respectively. Detection by PCR declined with days post-onset for influenza virus; decrease was faster for community-ARI than for inpatient-ARI. No such patterns were observed for non-influenza respiratory virus infections. PathChip added substantially to viruses detected for community-ARI only. Clinical case definitions discriminated influenza from other mild-ARI but performed poorly for severe-ARI and for older participants. Rational strategies for diagnosis and surveillance of influenza and other respiratory virus must acknowledge the differences between ARIs presenting in community and hospital settings. © 2017 The Author(s).
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/173940
ISSN: 20452322
DOI: 10.1038/srep42963
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