Please use this identifier to cite or link to this item: https://doi.org/10.7554/eLife.21064
Title: PCGF6-PRC1 suppresses premature differentiation of mouse embryonic stem cells by regulating germ cell-related genes
Authors: Endoh, M 
Endo, T.A
Shinga, J
Hayashi, K
Farcas, A
Ma, K.-W
Ito, S
Sharif, J
Endoh, T
Onaga, N
Nakayama, M
Ishikura, T
Masui, O
Kessler, B.M
Suda, T 
Ohara, O
Okuda, A
Klose, R
Koseki, H
Keywords: BMI1 protein
Max protein
melengestrol acetate
peptides and proteins
polycomb group ring finger protein 6
RING finger protein
transcription factor Dp1
transcription factor E2F6
transcription factor YAF2
unclassified drug
WDR5 protein
BMI1 protein
histone
Pcgf6 protein, mouse
Rnf2 protein, mouse
ubiquitin protein ligase
animal cell
animal tissue
Article
cell differentiation
cell growth
cell viability
controlled study
electroporation
embryo development
embryonic stem cell
gene control
gene expression
gene ontology
gene repression
gene silencing
gene targeting
histology
human
human cell
immunoprecipitation
mouse
nonhuman
protein interaction
reverse transcription polymerase chain reaction
upregulation
Western blotting
animal
embryonic stem cell
gene expression regulation
metabolism
physiology
Animals
Cell Differentiation
Embryonic Stem Cells
Gene Expression Regulation
Gene Silencing
Histones
Mice
Polycomb Repressive Complex 1
Ubiquitin-Protein Ligases
Issue Date: 2017
Citation: Endoh, M, Endo, T.A, Shinga, J, Hayashi, K, Farcas, A, Ma, K.-W, Ito, S, Sharif, J, Endoh, T, Onaga, N, Nakayama, M, Ishikura, T, Masui, O, Kessler, B.M, Suda, T, Ohara, O, Okuda, A, Klose, R, Koseki, H (2017). PCGF6-PRC1 suppresses premature differentiation of mouse embryonic stem cells by regulating germ cell-related genes. eLife 6 : e21064. ScholarBank@NUS Repository. https://doi.org/10.7554/eLife.21064
Abstract: The ring finger protein PCGF6 (polycomb group ring finger 6) interacts with RING1A/B and E2F6 associated factors to form a non-canonical PRC1 (polycomb repressive complex 1) known as PCGF6-PRC1. Here, we demonstrate that PCGF6-PRC1 plays a role in repressing a subset of PRC1 target genes by recruiting RING1B and mediating downstream mono-ubiquitination of histone H2A. PCGF6-PRC1 bound loci are highly enriched for promoters of germ cell-related genes in mouse embryonic stem cells (ESCs). Conditional ablation of Pcgf6 in ESCs leads to robust derepression of such germ cell-related genes, in turn affecting cell growth and viability. We also find a role for PCGF6 in pre- and peri-implantation mouse embryonic development. We further show that a heterodimer of the transcription factors MAX and MGA recruits PCGF6 to target loci. PCGF6 thus links sequence specific target recognition by the MAX/MGA complex to PRC1-dependent transcriptional silencing of germ cell-specific genes in pluripotent stem cells. © Endoh et al.
Source Title: eLife
URI: https://scholarbank.nus.edu.sg/handle/10635/173932
ISSN: 2050084X
DOI: 10.7554/eLife.21064
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