Please use this identifier to cite or link to this item: https://doi.org/10.1186/s12879-017-2427-4
Title: Valine/isoleucine variants drive selective pressure in the VP1 sequence of EV-A71 enteroviruses
Authors: Duy, N.N
Huong, L.T.T
Ravel, P
Huong, L.T.S
Dwivedi, A
Sessions, O.M 
Hou, Y.A 
Chua, R 
Kister, G
Afelt, A
Moulia, C
Gubler, D.J 
Thiem, V.D
Thanh, N.T.H
Devaux, C
Duong, T.N
Hien, N.T
Cornillot, E
Gavotte, L
Frutos, R
Keywords: isoleucine
protein VP1
valine
capsid protein
isoleucine
valine
VP1 protein, Foot-and-mouth disease virus
age
Article
child
controlled study
correlational study
correspondence analysis
disease severity
DNA polymorphism
Enterovirus A71
ethnicity
factorial correspondence analysis
female
gene mutation
gene sequence
genetic analysis
genetic variability
human
infant
major clinical study
male
nonhuman
nucleotide sequence
phylogeny
preschool child
school child
sequence alignment
spatiotemporal analysis
Viet Nam
virus gene
VP1 gene
Enterovirus
Enterovirus A
epidemic
genetic polymorphism
genetic selection
genetics
Hand, Foot and Mouth Disease
isolation and purification
mutation
pathogenicity
virology
Capsid Proteins
Child, Preschool
Disease Outbreaks
Enterovirus
Enterovirus A, Human
Epidemics
Female
Hand, Foot and Mouth Disease
Humans
Infant
Isoleucine
Male
Mutation
Phylogeny
Polymorphism, Genetic
Selection, Genetic
Spatio-Temporal Analysis
Valine
Vietnam
Issue Date: 2017
Publisher: BioMed Central Ltd.
Citation: Duy, N.N, Huong, L.T.T, Ravel, P, Huong, L.T.S, Dwivedi, A, Sessions, O.M, Hou, Y.A, Chua, R, Kister, G, Afelt, A, Moulia, C, Gubler, D.J, Thiem, V.D, Thanh, N.T.H, Devaux, C, Duong, T.N, Hien, N.T, Cornillot, E, Gavotte, L, Frutos, R (2017). Valine/isoleucine variants drive selective pressure in the VP1 sequence of EV-A71 enteroviruses. BMC Infectious Diseases 17 (1) : 333. ScholarBank@NUS Repository. https://doi.org/10.1186/s12879-017-2427-4
Abstract: Background: In 2011-2012, Northern Vietnam experienced its first large scale hand foot and mouth disease (HFMD) epidemic. In 2011, a major HFMD epidemic was also reported in South Vietnam with fatal cases. This 2011-2012 outbreak was the first one to occur in North Vietnam providing grounds to study the etiology, origin and dynamic of the disease. We report here the analysis of the VP1 gene of strains isolated throughout North Vietnam during the 2011-2012 outbreak and before. Methods: The VP1 gene of 106 EV-A71 isolates from North Vietnam and 2 from Central Vietnam were sequenced. Sequence alignments were analyzed at the nucleic acid and protein level. Gene polymorphism was also analyzed. A Factorial Correspondence Analysis was performed to correlate amino acid mutations with clinical parameters. Results: The sequences were distributed into four phylogenetic clusters. Three clusters corresponded to the subgenogroup C4 and the last one corresponded to the subgenogroup C5. Each cluster displayed different polymorphism characteristics. Proteins were highly conserved but three sites bearing only Isoleucine (I) or Valine (V) were characterized. The isoleucine/valine variability matched the clusters. Spatiotemporal analysis of the I/V variants showed that all variants which emerged in 2011 and then in 2012 were not the same but were all present in the region prior to the 2011-2012 outbreak. Some correlation was found between certain I/V variants and ethnicity and severity. Conclusions: The 2011-2012 outbreak was not caused by an exogenous strain coming from South Vietnam or elsewhere but by strains already present and circulating at low level in North Vietnam. However, what triggered the outbreak remains unclear. A selective pressure is applied on I/V variants which matches the genetic clusters. I/V variants were shown on other viruses to correlate with pathogenicity. This should be investigated in EV-A71. I/V variants are an easy and efficient way to survey and identify circulating EV-A71 strains. © 2017 The Author(s).
Source Title: BMC Infectious Diseases
URI: https://scholarbank.nus.edu.sg/handle/10635/173846
ISSN: 14712334
DOI: 10.1186/s12879-017-2427-4
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1186_s12879-017-2427-4.pdf1.76 MBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

3
checked on Jul 22, 2021

Page view(s)

69
checked on Jul 23, 2021

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.