Please use this identifier to cite or link to this item:
Title: Metabolic gene expression profile in circulating mononuclear cells reflects obesity-associated metabolic inflexibility
Authors: Baig, S 
Parvaresh Rizi, E
Shabeer, M 
Chhay, V 
Mok, S.F 
Loh, T.P 
Magkos, F 
Vidal-Puig, A
Tai, E.S 
Khoo, C.M 
Toh, S.-A 
Keywords: 6 phosphofructokinase
peroxisome proliferator activated receptor alpha
pyruvate dehydrogenase kinase 4
aerobic metabolism
chemical composition
clinical article
controlled study
disease association
fatty acid metabolism
gene expression profiling
gene induction
glucose oxidation
human cell
insulin resistance
lean body weight
mononuclear cell
respiratory quotient
transcription regulation
Issue Date: 2016
Citation: Baig, S, Parvaresh Rizi, E, Shabeer, M, Chhay, V, Mok, S.F, Loh, T.P, Magkos, F, Vidal-Puig, A, Tai, E.S, Khoo, C.M, Toh, S.-A (2016). Metabolic gene expression profile in circulating mononuclear cells reflects obesity-associated metabolic inflexibility. Nutrition and Metabolism 13 (1) : 1-Aug. ScholarBank@NUS Repository.
Abstract: Background: Obesity is associated with an impaired ability to switch from fatty acid to glucose oxidation during the fasted to fed transition, particularly in skeletal muscle. However, whether such metabolic inflexibility is reflected at the gene transcription level in circulatory mononuclear cells (MNC) is not known. Methods: The whole-body respiratory quotient (RQ) and transcriptional regulation of genes involved in carbohydrate and lipid metabolism in MNC were measured during fasting and in response (up to 6 h) to high-carbohydrate and high-fat meals in nine lean insulin-sensitive and nine obese insulin-resistant men. Results: Compared to lean subjects, obese subjects had an impaired ability to increase RQ and switch from fatty acid to glucose oxidation following the high-carbohydrate meal (interaction term P < 0.05). This was accompanied by an impaired induction of genes involved in oxidative metabolism of glucose in MNC, such as phosphofructokinase (PFK), pyruvate dehydrogenase kinase 4 (PDK4), peroxisome proliferator-activated receptor alpha (PPAR?) and uncoupling protein 3 (UCP3) and increased expression of genes involved in fatty acid metabolism, such as fatty acid translocase (FAT/CD36) and fatty acid synthase (FASN) (P < 0.05). On the contrary, there were no differences in the gene expression profiles between lean and obese subjects following the high-fat meal. Conclusions: Postprandial expression profiles of genes involved in glucose and fatty acid metabolism in the MNC reflect the differing metabolic flexibility phenotypes of our cohort of lean and obese individuals. These differences in metabolic flexibility between the lean and obese are elicited by an acute meal challenge that is rich in carbohydrate but not fat. © 2016 The Author(s).
Source Title: Nutrition and Metabolism
ISSN: 17437075
DOI: 10.1186/s12986-016-0135-5
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1186_s12986-016-0135-5.pdf815.74 kBAdobe PDF




checked on Nov 22, 2020

Page view(s)

checked on Nov 20, 2020

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.