Please use this identifier to cite or link to this item: https://doi.org/10.1186/s13045-017-0532-x
DC FieldValue
dc.titleAn improved pre-clinical patient-derived liquid xenograft mouse model for acute myeloid leukemia
dc.contributor.authorHer Z.
dc.contributor.authorYong K.S.M.
dc.contributor.authorParamasivam K.
dc.contributor.authorTan W.W.S.
dc.contributor.authorChan X.Y.
dc.contributor.authorTan S.Y.
dc.contributor.authorLiu M.
dc.contributor.authorFan Y.
dc.contributor.authorLinn Y.C.
dc.contributor.authorHui K.M.
dc.contributor.authorSurana U.
dc.contributor.authorChen Q.
dc.date.accessioned2020-09-01T00:50:41Z
dc.date.available2020-09-01T00:50:41Z
dc.date.issued2017
dc.identifier.citationHer Z., Yong K.S.M., Paramasivam K., Tan W.W.S., Chan X.Y., Tan S.Y., Liu M., Fan Y., Linn Y.C., Hui K.M., Surana U., Chen Q. (2017). An improved pre-clinical patient-derived liquid xenograft mouse model for acute myeloid leukemia. Journal of Hematology and Oncology 10 (1) : 162. ScholarBank@NUS Repository. https://doi.org/10.1186/s13045-017-0532-x
dc.identifier.issn17568722
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/173769
dc.description.abstractBackground: Xenotransplantation of patient-derived AML (acute myeloid leukemia) cells in NOD-scid Il2r? null (NSG) mice is the method of choice for evaluating this human hematologic malignancy. However, existing models constructed using intravenous injection in adult or newborn NSG mice have inferior engraftment efficiency, poor peripheral blood engraftment, or are difficult to construct. Methods: Here, we describe an improved AML xenograft model where primary human AML cells were injected into NSG newborn pups intrahepatically. Results: Introduction of primary cells from AML patients resulted in high levels of engraftment in peripheral blood, spleen, and bone marrow (BM) of recipient mice. The phenotype of engrafted AML cells remained unaltered during serial transplantation. The mice developed features that are consistent with human AML including spleen enlargement and infiltration of AML cells into multiple organs. Importantly, we demonstrated that although leukemic stem cell activity is enriched and mediated by CD34+CD117+ subpopulation, CD34+CD117- subpopulation can acquire CD34+CD117+ phenotype through de-differentiation. Lastly, we evaluated the therapeutic potential of Sorafenib and Regorafenib in this AML model and found that periphery and spleen AML cells are sensitive to these treatments, whereas BM provides a protective environment to AML. Conclusions: Collectively, our improved model is robust, easy-to-construct, and reliable for pre-clinical AML studies. © 2017 The Author(s).
dc.sourceUnpaywall 20200831
dc.subjectregorafenib
dc.subjectsorafenib
dc.subjectstem cell factor receptor
dc.subjectacute myeloid leukemia
dc.subjectacute myeloid leukemia cell line
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectblood analysis
dc.subjectbone marrow
dc.subjectCD34 selection
dc.subjectcell activity
dc.subjectcell differentiation
dc.subjectcell infiltration
dc.subjectcell subpopulation
dc.subjectdisease course
dc.subjectdrug screening
dc.subjectdrug sensitivity
dc.subjectengraftment
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmouse
dc.subjectnonhuman
dc.subjectphenotype
dc.subjectpreclinical study
dc.subjectprimary cell line
dc.subjectspleen
dc.subjectsplenomegaly
dc.subjecttherapy effect
dc.subjecttransplantation
dc.subjecttumor xenograft
dc.subjectacute myeloid leukemia
dc.subjectanimal
dc.subjectdisease model
dc.subjectgenetics
dc.subjectnonobese diabetic mouse
dc.subjectpathology
dc.subjectprocedures
dc.subjectSCID mouse
dc.subjectxenograft
dc.subjectAnimals
dc.subjectDisease Models, Animal
dc.subjectHumans
dc.subjectLeukemia, Myeloid, Acute
dc.subjectMice
dc.subjectMice, Inbred NOD
dc.subjectMice, SCID
dc.subjectTransplantation, Heterologous
dc.typeArticle
dc.contributor.departmentDEPT OF MEDICINE
dc.contributor.departmentDEPT OF PHARMACOLOGY
dc.contributor.departmentDEPT OF MICROBIOLOGY & IMMUNOLOGY
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.doi10.1186/s13045-017-0532-x
dc.description.sourcetitleJournal of Hematology and Oncology
dc.description.volume10
dc.description.issue1
dc.description.page162
Appears in Collections:Staff Publications
Elements

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1186_s13045-017-0532-x.pdf4.81 MBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

13
checked on Jul 21, 2021

Page view(s)

77
checked on Jul 23, 2021

Download(s)

1
checked on Jul 23, 2021

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.