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Title: NLRP10 enhances CD4+ T-cell-mediated IFN? response via regulation of dendritic cell-derived IL-12 release
Authors: Vacca, M 
Böhme, J
Zambetti, L.P
Khameneh, H.J
Paleja, B.S
Laudisi, F
Ho, A.W.S
Neo, K
Leong, K.W.K
Marzuki, M
Lee, B
Poidinger, M 
Santambrogio, L
Tsenova, L
Zolezzi, F
de Libero, G
Singhal, A
Mortellaro, A 
Keywords: CD134 antigen
CD137 antigen
CD40 antigen
gamma interferon
Hermes antigen
immunoglobulin enhancer binding protein
interleukin 12
interleukin 12p40
interleukin 12p70
interleukin 6
membrane protein
toll like receptor 9
tumor necrosis factor
unclassified drug
animal cell
animal experiment
animal tissue
bacterial growth
CD4+ T lymphocyte
cell differentiation
cell proliferation
cell proliferation assay
cell stimulation
colony forming unit
controlled study
cytokine production
dendritic cell
enzyme linked immunosorbent assay
flow cytometry
immune response
intracellular signaling
Mycobacterium tuberculosis
protein expression
regulatory mechanism
RNA isolation
Issue Date: 2017
Citation: Vacca, M, Böhme, J, Zambetti, L.P, Khameneh, H.J, Paleja, B.S, Laudisi, F, Ho, A.W.S, Neo, K, Leong, K.W.K, Marzuki, M, Lee, B, Poidinger, M, Santambrogio, L, Tsenova, L, Zolezzi, F, de Libero, G, Singhal, A, Mortellaro, A (2017). NLRP10 enhances CD4+ T-cell-mediated IFN? response via regulation of dendritic cell-derived IL-12 release. Frontiers in Immunology 8 (NOV) : 1462. ScholarBank@NUS Repository.
Abstract: NLRP10 is a nucleotide-binding oligomerization domain-like receptor that functions as an intracellular pattern recognition receptor for microbial products. Here, we generated a Nlrp10-/- mouse to delineate the role of NLRP10 in the host immune response and found that Nlrp10-/- dendritic cells (DCs) elicited sub-optimal IFN? production by antigen-specific CD4+ T cells compared to wild-type (WT) DCs. In response to T-cell encounter, CD40 ligation or Toll-like receptor 9 stimulation, Nlrp10-/- DCs produced low levels of IL-12, due to a substantial decrease in NF-?B activation. Defective IL-12 production was also evident in vivo and affected IFN? production by CD4+ T cells. Upon Mycobacterium tuberculosis (Mtb) infection, Nlrp10-/- mice displayed diminished T helper 1-cell responses and increased bacterial growth compared to WT mice. These data indicate that NLRP10-mediated IL-12 production by DCs is critical for IFN? induction in T cells and contributes to promote the host defense against Mtb. © 2017 Vacca, Böhme, Zambetti, Khameneh, Paleja, Laudisi, Ho, Neo, Leong, Marzuki, Lee, Poidinger, Santambrogio, Tsenova, Zolezzi, De Libero, Singhal and Mortellaro.
Source Title: Frontiers in Immunology
ISSN: 16643224
DOI: 10.3389/fimmu.2017.01462
Appears in Collections:Staff Publications

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