Please use this identifier to cite or link to this item: https://doi.org/10.1096/fj.15-275396
Title: Transient receptor potential vanilloid 2-mediated shear-stress responses in C2C12 myoblasts are regulated by serum and extracellular matrix
Authors: Kurth, Felix
Franco-Obregon, Alfredo 
Casarosa, Marco
Kuester, Simon K
Wuertz-Kozak, Karin
Dittrich, Petra S
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Biology
Cell Biology
Life Sciences & Biomedicine - Other Topics
mechanotransduction
calcium
laminin
myogenesis
microfluidics
MECHANOSENSITIVE ION CHANNELS
VASCULAR ENDOTHELIAL-CELLS
OPERATED CALCIUM-CHANNELS
PATCH-CLAMPED MEMBRANES
SKELETAL-MUSCLE FIBERS
TRP CHANNELS
MECHANICAL STIMULATION
MUSCULAR-DYSTROPHY
CATION CHANNELS
2-AMINOETHOXYDIPHENYL BORATE
Issue Date: 1-Nov-2015
Publisher: FEDERATION AMER SOC EXP BIOL
Citation: Kurth, Felix, Franco-Obregon, Alfredo, Casarosa, Marco, Kuester, Simon K, Wuertz-Kozak, Karin, Dittrich, Petra S (2015-11-01). Transient receptor potential vanilloid 2-mediated shear-stress responses in C2C12 myoblasts are regulated by serum and extracellular matrix. FASEB JOURNAL 29 (11) : 4726-4737. ScholarBank@NUS Repository. https://doi.org/10.1096/fj.15-275396
Abstract: © FASEB. The developmental sensitivity of skeletal muscle to mechanical forces is unparalleled in other tissues. Calcium entry via reputedly mechanosensitive transient receptor potential (TRP) channel classes has been shown to play an essential role in both the early proliferative stage and subsequent differentiation of skeletal muscle myoblasts, particularly TRP canonical (TRPC) 1 and TRP vanilloid (TRPV) 2. Here we show that C2C12 murine myoblasts respond to fluid flow-induced shear stress with increments in cytosolic calcium that are largely initiated by the mechanosensitive opening of TRPV2 channels.Response to fluid flowwas augmented by growth in low extracellular serum concentration (5 vs. 20% fetal bovine serum) by greater than 9-fold and at 18 h in culture, coincident with the greatest TRPV2 channel expression under identical conditions (P < 0.02). Fluid flow responses were also enhanced by substrate functionalization with laminin, rather than with fibronectin, agreeing with previous findings that the gating of TRPV2 is facilitated by laminin. Fluid flow-induced calcium increments were blocked by ruthenium red (27%) and SKF-96365 (38%), whereas they were unaltered by 2-aminoethoxydiphenyl borate, further corroborating that TRPV2 channels play a predominant role in fluid flow mechanosensitivity over that ofTRPC1 andTRPmelastatin (TRPM) 7.-Kurth, F., Franco-Obregón, A., Casarosa, M., Küster, S. K., Wuertz-Kozak, K., Dittrich, P. S. Transient receptor potential vanilloid 2-mediated shear-stress responses in C2C12 myoblasts are regulated by serum and extracellular matrix.
Source Title: FASEB JOURNAL
URI: https://scholarbank.nus.edu.sg/handle/10635/173640
ISSN: 08926638
15306860
DOI: 10.1096/fj.15-275396
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