Please use this identifier to cite or link to this item:
https://doi.org/10.1038/s41592-020-0858-0
DC Field | Value | |
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dc.title | Highly specific multiplexed RNA imaging in tissues with split-FISH | |
dc.contributor.author | Goh, Jolene Jie Lin | |
dc.contributor.author | Chou, Nigel | |
dc.contributor.author | Seow, Wan Yi | |
dc.contributor.author | Ha, Norbert | |
dc.contributor.author | Cheng, Chung Pui Paul | |
dc.contributor.author | Chang, Yun-Ching | |
dc.contributor.author | Zhao, Ziqing Winston | |
dc.contributor.author | Chen, Kok Hao | |
dc.date.accessioned | 2020-08-25T03:31:38Z | |
dc.date.available | 2020-08-25T03:31:38Z | |
dc.date.issued | 2020-06-15 | |
dc.identifier.citation | Goh, Jolene Jie Lin, Chou, Nigel, Seow, Wan Yi, Ha, Norbert, Cheng, Chung Pui Paul, Chang, Yun-Ching, Zhao, Ziqing Winston, Chen, Kok Hao (2020-06-15). Highly specific multiplexed RNA imaging in tissues with split-FISH. NATURE METHODS 17 (7). ScholarBank@NUS Repository. https://doi.org/10.1038/s41592-020-0858-0 | |
dc.identifier.issn | 15487091 | |
dc.identifier.issn | 15487105 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/173463 | |
dc.description.abstract | © 2020, The Author(s), under exclusive licence to Springer Nature America, Inc. We present split-FISH, a multiplexed fluorescence in situ hybridization method that leverages a split-probe design to achieve enhanced specificity. Split-FISH reduces off-target background fluorescence, decreases false positives and enables accurate RNA profiling in uncleared tissues. We demonstrate the efficacy of split-FISH on various mouse tissues by quantifying the distribution and abundance of 317 genes in single cells and reveal diverse localization patterns for spatial regulation of the transcriptome in complex tissues. | |
dc.language.iso | en | |
dc.publisher | NATURE PUBLISHING GROUP | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Life Sciences & Biomedicine | |
dc.subject | Biochemical Research Methods | |
dc.subject | Biochemistry & Molecular Biology | |
dc.type | Article | |
dc.date.updated | 2020-07-19T08:46:21Z | |
dc.contributor.department | DEPT OF CHEMISTRY | |
dc.contributor.department | DEPT OF MEDICINE | |
dc.description.doi | 10.1038/s41592-020-0858-0 | |
dc.description.sourcetitle | NATURE METHODS | |
dc.description.volume | 17 | |
dc.description.issue | 7 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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File | Description | Size | Format | Access Settings | Version | |
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s41592-020-0858-0.pdf | Published version | 20.9 MB | Adobe PDF | CLOSED | None |
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