Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41592-020-0858-0
Title: Highly specific multiplexed RNA imaging in tissues with split-FISH
Authors: Goh, Jolene Jie Lin
Chou, Nigel
Seow, Wan Yi
Ha, Norbert
Cheng, Chung Pui Paul 
Chang, Yun-Ching
Zhao, Ziqing Winston 
Chen, Kok Hao
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Biochemistry & Molecular Biology
Issue Date: 15-Jun-2020
Publisher: NATURE PUBLISHING GROUP
Citation: Goh, Jolene Jie Lin, Chou, Nigel, Seow, Wan Yi, Ha, Norbert, Cheng, Chung Pui Paul, Chang, Yun-Ching, Zhao, Ziqing Winston, Chen, Kok Hao (2020-06-15). Highly specific multiplexed RNA imaging in tissues with split-FISH. NATURE METHODS 17 (7). ScholarBank@NUS Repository. https://doi.org/10.1038/s41592-020-0858-0
Abstract: © 2020, The Author(s), under exclusive licence to Springer Nature America, Inc. We present split-FISH, a multiplexed fluorescence in situ hybridization method that leverages a split-probe design to achieve enhanced specificity. Split-FISH reduces off-target background fluorescence, decreases false positives and enables accurate RNA profiling in uncleared tissues. We demonstrate the efficacy of split-FISH on various mouse tissues by quantifying the distribution and abundance of 317 genes in single cells and reveal diverse localization patterns for spatial regulation of the transcriptome in complex tissues.
Source Title: NATURE METHODS
URI: https://scholarbank.nus.edu.sg/handle/10635/173463
ISSN: 15487091
15487105
DOI: 10.1038/s41592-020-0858-0
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