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https://doi.org/10.1007/978-1-4939-3618-2_9
| Title: | Infectious viral quantification of chikungunya virus—virus plaque assay | Authors: | Kaur, P Lee, RCH Chu, JJH |
Keywords: | Aquacide Carboxymethyl cellulose Chikungunya virus Crystal violet Plaque assays Virus titration Animals Cell Line Chikungunya virus Viral Load Viral Plaque Assay |
Issue Date: | 1-Jan-2016 | Publisher: | Springer New York | Citation: | Kaur, P, Lee, RCH, Chu, JJH (2016-01-01). Infectious viral quantification of chikungunya virus—virus plaque assay. Methods Mol Biol 1426 : 93-103. ScholarBank@NUS Repository. https://doi.org/10.1007/978-1-4939-3618-2_9 | Abstract: | © Springer Science+Business Media New York 2016. The plaque assay is an essential method for quantification of infectious virus titer. Cells infected with virus particles are overlaid with a viscous substrate. A suitable incubation period results in the formation of plaques, which can be fixed and stained for visualization. Here, we describe a method for measuring Chikungunya virus (CHIKV) titers via virus plaque assays. | Source Title: | Methods Mol Biol | URI: | https://scholarbank.nus.edu.sg/handle/10635/173287 | ISSN: | 10643745 19406029 |
DOI: | 10.1007/978-1-4939-3618-2_9 |
| Appears in Collections: | Staff Publications Elements |
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| 22.pdf | Accepted version | 435.98 kB | Adobe PDF | OPEN | Post-print | View/Download |
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