Please use this identifier to cite or link to this item: https://doi.org/10.1021/acsami.5b03766
Title: Biocompatible Green and Red Fluorescent Organic Dots with Remarkably Large Two-Photon Action Cross Sections for Targeted Cellular Imaging and Real-Time Intravital Blood Vascular Visualization
Authors: Xiang, Jiayun
Cai, Xiaolei 
Lou, Xiaoding
Feng, Guangxue 
Min, Xuehong
Luo, Wenwen
He, Bairong
Goh, Chi Ching 
Ng, Lai Guan 
Zhou, Jian
Zhao, Zujin
Liu, Bin 
Tang, Ben Zhong
Keywords: Science & Technology
Technology
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Science & Technology - Other Topics
Materials Science
organic dots
two-photon absorption
two-photon fluorescence
bioimaging
tetraphenylethene
AGGREGATION-INDUCED EMISSION
CONJUGATED POLYMER NANOPARTICLES
LONG-TERM
SILICA NANOPARTICLES
QUANTUM DOTS
STEM-CELLS
AIE DOTS
TETRAPHENYLETHENE
PROBES
CYTOTOXICITY
Issue Date: 15-Jul-2015
Publisher: AMER CHEMICAL SOC
Citation: Xiang, Jiayun, Cai, Xiaolei, Lou, Xiaoding, Feng, Guangxue, Min, Xuehong, Luo, Wenwen, He, Bairong, Goh, Chi Ching, Ng, Lai Guan, Zhou, Jian, Zhao, Zujin, Liu, Bin, Tang, Ben Zhong (2015-07-15). Biocompatible Green and Red Fluorescent Organic Dots with Remarkably Large Two-Photon Action Cross Sections for Targeted Cellular Imaging and Real-Time Intravital Blood Vascular Visualization. ACS APPLIED MATERIALS & INTERFACES 7 (27) : 14965-14974. ScholarBank@NUS Repository. https://doi.org/10.1021/acsami.5b03766
Abstract: © 2015 American Chemical Society. Fluorescent organic dots are emerging as promising bioimaging reagents because of their high brightness, good photostability, excellent biocompatibility, and facile surface functionalization. Organic dots with large two-photon absorption (TPA) cross sections are highly desired for two-photon fluorescence microscopy. In this work, we report two biocompatible and photostable organic dots fabricated by encapsulating tetraphenylethene derivatives within DSPE-PEG matrix. The two organic dots show absorption maxima at 425 and 483 nm and emit green and red fluorescence at 560 and 645 nm, with high fluorescence quantum yields of 64% and 22%, respectively. Both organic dots exhibit excellent TPA property in the range of 800-960 nm, affording upon excitation at 820 nm remarkably large TPA cross sections of 1.2 × 106 and 2.5 × 106 GM on the basis of dot concentration. The bare fluorophores and their organic dots are biocompatible and have been used to stain living cells for one- and two-photon fluorescence bioimagings. The cRGD-modified organic dots can selectively target integrin αvβ3 overexpressing breast cancer cells for targeted imaging. The organic dots are also applied for real-time two-photon fluorescence in vivo visualization of the blood vasculature of mouse ear, providing the spatiotemporal information about the whole blood vascular network. These results demonstrate that the present fluorescent organic dots are promising candidates for living cell and tissue imaging.
Source Title: ACS APPLIED MATERIALS & INTERFACES
URI: https://scholarbank.nus.edu.sg/handle/10635/171899
ISSN: 19448244
19448252
DOI: 10.1021/acsami.5b03766
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