Please use this identifier to cite or link to this item:
https://doi.org/10.1021/acs.analchem.6b00023
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dc.title | Peptide-Induced AIEgen Self-Assembly: A New Strategy to Realize Highly Sensitive Fluorescent Light-Up Probes | |
dc.contributor.author | Han, Aitian | |
dc.contributor.author | Wang, Huaimin | |
dc.contributor.author | Kwok, Ryan TK | |
dc.contributor.author | Ji, Shenglu | |
dc.contributor.author | Li, Jun | |
dc.contributor.author | Kong, Deling | |
dc.contributor.author | Tang, Ben Zhong | |
dc.contributor.author | Liu, Bin | |
dc.contributor.author | Yang, Zhimou | |
dc.contributor.author | Ding, Dan | |
dc.date.accessioned | 2020-08-04T07:50:36Z | |
dc.date.available | 2020-08-04T07:50:36Z | |
dc.date.issued | 2016-04-05 | |
dc.identifier.citation | Han, Aitian, Wang, Huaimin, Kwok, Ryan TK, Ji, Shenglu, Li, Jun, Kong, Deling, Tang, Ben Zhong, Liu, Bin, Yang, Zhimou, Ding, Dan (2016-04-05). Peptide-Induced AIEgen Self-Assembly: A New Strategy to Realize Highly Sensitive Fluorescent Light-Up Probes. ANALYTICAL CHEMISTRY 88 (7) : 3872-3878. ScholarBank@NUS Repository. https://doi.org/10.1021/acs.analchem.6b00023 | |
dc.identifier.issn | 00032700 | |
dc.identifier.issn | 15206882 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/171893 | |
dc.description.abstract | © 2016 American Chemical Society. Fluorescent light-up probes with aggregation-induced emission (AIE) characteristics have recently attracted great research interest due to their intelligent fluorescence activation mechanism and excellent photobleaching resistance. In this work, we report a new, simple, and generic strategy to design and prepare highly sensitive AIE fluorescent light-up bioprobe through facile incorporation of a self-assembling peptide sequence GFFY between the recognition element and the AIE luminogen (AIEgen). After the bioprobes respond to the targets, the peptide GFFY is capable of inducing the ordered self-assembly of AIEgens, yielding close and tight intermolecular steric interactions to restrict the intramolecular motions of AIEgens for excellent signal output. Using two proof-of-concepts, we have demonstrated that self-assembling peptide-incorporating AIE light-up probes show much higher sensitivity in sensing the corresponding targets in both solutions and cancer cells as compared to those without GFFY induced self-assembly. Taking the probe TPE-GFFYK(DVEDEE-Ac), for example, a detection limit as low as 0.54 pM can be achieved for TPE-GFFYK(DVEDEE-Ac) in caspase-3 detection, which is much lower than that of TPE-K(DVED-Ac) (3.50 pM). This study may inspire new insights into the design of advanced fluorescent molecular probes. (Figure Presented). | |
dc.language.iso | en | |
dc.publisher | AMER CHEMICAL SOC | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Physical Sciences | |
dc.subject | Chemistry, Analytical | |
dc.subject | Chemistry | |
dc.subject | AGGREGATION-INDUCED EMISSION | |
dc.subject | TURN-ON | |
dc.subject | HYDROGEN-PEROXIDE | |
dc.subject | CELL APOPTOSIS | |
dc.subject | CANCER-CELLS | |
dc.subject | PROTEIN | |
dc.subject | NANOPROBES | |
dc.subject | MOLECULES | |
dc.subject | BIOPROBE | |
dc.subject | ENZYME | |
dc.type | Article | |
dc.date.updated | 2020-08-04T07:38:04Z | |
dc.contributor.department | CHEMICAL & BIOMOLECULAR ENGINEERING | |
dc.description.doi | 10.1021/acs.analchem.6b00023 | |
dc.description.sourcetitle | ANALYTICAL CHEMISTRY | |
dc.description.volume | 88 | |
dc.description.issue | 7 | |
dc.description.page | 3872-3878 | |
dc.published.state | Published | |
Appears in Collections: | Elements Staff Publications |
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Peptide-Induced AIEgen Self-Assembly A New Strategy to Realize Highly Sensitive Fluorescent Light-Up Probes.pdf | Accepted version | 500.9 kB | Adobe PDF | OPEN | Post-print | View/Download |
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