Please use this identifier to cite or link to this item: https://doi.org/10.1021/acs.analchem.6b00023
Title: Peptide-Induced AIEgen Self-Assembly: A New Strategy to Realize Highly Sensitive Fluorescent Light-Up Probes
Authors: Han, Aitian
Wang, Huaimin
Kwok, Ryan TK
Ji, Shenglu
Li, Jun
Kong, Deling
Tang, Ben Zhong
Liu, Bin 
Yang, Zhimou
Ding, Dan 
Keywords: Science & Technology
Physical Sciences
Chemistry, Analytical
Chemistry
AGGREGATION-INDUCED EMISSION
TURN-ON
HYDROGEN-PEROXIDE
CELL APOPTOSIS
CANCER-CELLS
PROTEIN
NANOPROBES
MOLECULES
BIOPROBE
ENZYME
Issue Date: 5-Apr-2016
Publisher: AMER CHEMICAL SOC
Citation: Han, Aitian, Wang, Huaimin, Kwok, Ryan TK, Ji, Shenglu, Li, Jun, Kong, Deling, Tang, Ben Zhong, Liu, Bin, Yang, Zhimou, Ding, Dan (2016-04-05). Peptide-Induced AIEgen Self-Assembly: A New Strategy to Realize Highly Sensitive Fluorescent Light-Up Probes. ANALYTICAL CHEMISTRY 88 (7) : 3872-3878. ScholarBank@NUS Repository. https://doi.org/10.1021/acs.analchem.6b00023
Abstract: © 2016 American Chemical Society. Fluorescent light-up probes with aggregation-induced emission (AIE) characteristics have recently attracted great research interest due to their intelligent fluorescence activation mechanism and excellent photobleaching resistance. In this work, we report a new, simple, and generic strategy to design and prepare highly sensitive AIE fluorescent light-up bioprobe through facile incorporation of a self-assembling peptide sequence GFFY between the recognition element and the AIE luminogen (AIEgen). After the bioprobes respond to the targets, the peptide GFFY is capable of inducing the ordered self-assembly of AIEgens, yielding close and tight intermolecular steric interactions to restrict the intramolecular motions of AIEgens for excellent signal output. Using two proof-of-concepts, we have demonstrated that self-assembling peptide-incorporating AIE light-up probes show much higher sensitivity in sensing the corresponding targets in both solutions and cancer cells as compared to those without GFFY induced self-assembly. Taking the probe TPE-GFFYK(DVEDEE-Ac), for example, a detection limit as low as 0.54 pM can be achieved for TPE-GFFYK(DVEDEE-Ac) in caspase-3 detection, which is much lower than that of TPE-K(DVED-Ac) (3.50 pM). This study may inspire new insights into the design of advanced fluorescent molecular probes. (Figure Presented).
Source Title: ANALYTICAL CHEMISTRY
URI: https://scholarbank.nus.edu.sg/handle/10635/171893
ISSN: 00032700
15206882
DOI: 10.1021/acs.analchem.6b00023
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