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https://scholarbank.nus.edu.sg/handle/10635/17100
DC Field | Value | |
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dc.title | Tissue engineering of an osteochondral transplant by using a cell/scaffold construct | |
dc.contributor.author | HO SAEY TUAN | |
dc.date.accessioned | 2010-05-13T19:32:24Z | |
dc.date.available | 2010-05-13T19:32:24Z | |
dc.date.issued | 2009-06-28 | |
dc.identifier.citation | HO SAEY TUAN (2009-06-28). Tissue engineering of an osteochondral transplant by using a cell/scaffold construct. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/17100 | |
dc.description.abstract | Traditional clinical remedies are unable to address osteochondral defects adequately.<br>Given the paucity of available alternatives, the author aims to harness the advances in<br>stem cell and biomaterial research to create a biphasic osteochondral implant that caters to<br>both cartilage and bone regeneration. The endeavor was driven by the hypothesis that a<br>biomechanically competent biphasic scaffold that is seeded with hydrogel encapsulated<br>Mesenchymal Stem Cells (MSC) would support osteochondral repair. Therefore the aim<br>would be to select a suitable cartilage hydrogel and to engineer scaffolds which are<br>mechanically compatible to the native osteochondral tissue. Moreover the design of a<br>cartilage resurfacing membrane constituted an additional objective. Lastly, the feasibility<br>of the assembled construct had to be validated in animal models. The investigation<br>proceeded with a cartilage hydrogel selection. Consequently, fibrin was found to enhance<br>MSC chondrogenesis, cellular growth and extracellular matrix synthesis in in vitro 3D<br>osteochondral constructs. This bioactive hydrogel was coupled with rapid prototyped<br>polycaprolactone b based scaffolds in the reconstruction of critically sized osteochondral<br>defects in rabbits. These scaffolds were sufficiently porous and they mimicked the<br>mechanical characteristics of bone and cartilage. In vivo findings indicated bone repair to be facilitated by the open architecture of the scaffolds while cartilage regeneration was<br>reliant on the implanted MSC and matrix support. However the unsatisfactory healing at<br>the cartilage surface suggested the inclusion of a membrane that would help to retain the<br>seeded cells. In that light, the use of polycaprolactone - collagen electrospun meshes were explored. The synthetic membrane demonstrated MSC compatibility in the in vitro chondrogenic environment without inducing a hypertropic response. All these findings have prompted a large animal study with translational objectives. Osteochondral healing in the large animal was enhanced by the use of the implanted MSC within the biphasic<br>scaffold and the electrospun mesh. However tissue healing was not just dependent on<br>exogenous factors but also on the endogenous biomechanical features at the defect site.<br>The research efforts have yielded a functional osteochondral implant with due attention<br>given to the specific components and the concept was validated in the final preclinical<br>model. | |
dc.language.iso | en | |
dc.subject | Tissue engineering, osteochondral, mesenchymal stem cell | |
dc.type | Thesis | |
dc.contributor.department | GRADUATE PROGRAMME IN BIOENGINEERING-SOM | |
dc.contributor.supervisor | GOH CHO HONG, JAMES | |
dc.contributor.supervisor | HUTMACHER, DIETMAR WERNER | |
dc.contributor.supervisor | SIMON MCKENZIE COOL | |
dc.contributor.supervisor | ROBERT E. GULBERG | |
dc.contributor.supervisor | HUI HOI PO | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | DOCTOR OF PHILOSOPHY | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Ph.D Theses (Open) |
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File | Description | Size | Format | Access Settings | Version | |
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Ho STB PhD thesis.pdf | 4.75 MB | Adobe PDF | OPEN | None | View/Download |
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