Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep12727
Title: Antiviral Phosphorodiamidate Morpholino Oligomers are Protective against Chikungunya Virus Infection on Cell-based and Murine Models
Authors: Lam, Shirley
CHEN HUIXIN 
Chen, Caiyun Karen
NYO MIN 
CHU JANG HANN 
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
MOUSE MODEL
INHIBITION
MICE
REPLICATION
EFFICACY
FEVER
TRANSLATION
CHLOROQUINE
RESISTANCE
ARTHRITIS
Issue Date: 30-Jul-2015
Publisher: NATURE PUBLISHING GROUP
Citation: Lam, Shirley, CHEN HUIXIN, Chen, Caiyun Karen, NYO MIN, CHU JANG HANN (2015-07-30). Antiviral Phosphorodiamidate Morpholino Oligomers are Protective against Chikungunya Virus Infection on Cell-based and Murine Models. SCIENTIFIC REPORTS 5 (1). ScholarBank@NUS Repository. https://doi.org/10.1038/srep12727
Abstract: Chikungunya virus (CHIKV) infection in human is associated with debilitating and persistent arthralgia and arthritis. Currently, there is no specific vaccine or effective antiviral available. Anti-CHIKV Phosphorodiamidate Morpholino Oligomer (CPMO) was evaluated for its antiviral efficacy and cytotoxcity in human cells and neonate murine model. Two CPMOs were designed to block translation initiation of a highly conserved sequence in CHIKV non-structural and structural polyprotein, respectively. Pre-treatment of HeLa cells with CPMO1 signficantly suppressed CHIKV titre, CHIKV E2 protein expression and prevented CHIKV-induced CPE. CPMO1 activity was also CHIKV-specific as shown by the lack of cross-reactivity against SINV or DENV replication. When administered prophylactically in neonate mice, 15â €‰Î 1/4g/g CPMO1v conferred 100% survival against CHIKV disease. In parallel, these mice demonstrated significant reduction in viremia and viral load in various tissues. Immunohistological examination of skeletal muscles and liver of CPMO1v-treated mice also showed healthy tissue morphology, in contrast to evident manifestation of CHIKV pathogenesis in PBS- or scrambled sCPMO1v-treated groups. Taken together, our findings highlight for the first time that CPMO1v has strong protective effect against CHIKV infection. This warrants future development of morpholino as an alternative antiviral agent to address CHIKV infection in clinical applications.
Source Title: SCIENTIFIC REPORTS
URI: https://scholarbank.nus.edu.sg/handle/10635/170690
ISSN: 2045-2322
DOI: 10.1038/srep12727
Appears in Collections:Staff Publications
Elements

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
srep12727.pdfPublished version6.39 MBAdobe PDF

OPEN

PublishedView/Download

SCOPUSTM   
Citations

9
checked on Aug 7, 2022

Page view(s)

171
checked on Aug 4, 2022

Download(s)

1
checked on Aug 4, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.