Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pntd.0007610
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dc.titleMacropinocytosis dependent entry of Chikungunya virus into human muscle cells
dc.contributor.authorLee, Ching Hua Regina
dc.contributor.authorHussain, Khairunnisa Mohamed
dc.contributor.authorChu, Justin Jang Hann
dc.date.accessioned2020-06-23T07:45:41Z
dc.date.available2020-06-23T07:45:41Z
dc.date.issued2019-08-01
dc.identifier.citationLee, Ching Hua Regina, Hussain, Khairunnisa Mohamed, Chu, Justin Jang Hann (2019-08-01). Macropinocytosis dependent entry of Chikungunya virus into human muscle cells. PLOS NEGLECTED TROPICAL DISEASES 13 (8). ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pntd.0007610
dc.identifier.issn19352735
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/170665
dc.description.abstract© 2019 Lee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Chikungunya virus (CHIKV) is a re-emerging arbovirus known to cause chronic myalgia and arthralgia with high morbidity. CHIKV is now considered endemic in many countries across Asia and Africa. In this study, the susceptibility of various human, mammalian and mosquito cell lines to CHIKV infection was evaluated. CHIKV infection was found to be cell-type dependent and virus strain-specific. Furthermore, SJCRH30 (human rhabdomyosarcoma cell line) was showed to be highly permissive to CHIKV infection, with maximum production of infectious virions observed at 12 h.p.i. Pre-infection treatment of SJCRH30 with various inhibitors of endocytosis, including monodansylcadaverine (receptor-mediated endocytic inhibitor), dynasore (clathrin-mediated endocytic inhibitor), as well as filipin (caveolin-mediated endocytosis inhibitor), resulted in minimal inhibition of CHIKV infection. In contrast, dose-dependent inhibition of CHIKV infection was observed with the treatment of macropinocytosis inhibitor, 5-(N-ethyl-N-isopropyl)amiloride (EIPA). Furthermore, siRNA-mediated knockdown of sortin nexin 9 (SNX9) a protein involved in macropinosome formation, also resulted in a significant dose-dependent reduction in viral titre. By performing a virus entry assay, CHIKV particles were also observed to colocalize with FITC-dextran, a macropinosome marker. This study shows for the first time, that the infectious entry of CHIKV into human muscle cells is mediated by macropinocytosis. Together, the data from this study may pave the way for the development of specific inhibitors that target the entry process of CHIKV into cells.
dc.language.isoen
dc.publisherPUBLIC LIBRARY SCIENCE
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectInfectious Diseases
dc.subjectParasitology
dc.subjectTropical Medicine
dc.subjectLOW PH
dc.subjectINFECTION
dc.subjectINTERNALIZATION
dc.subjectPENETRATION
dc.subjectINHIBITION
dc.subjectEXPOSURE
dc.subjectCAVEOLIN
dc.subjectSURFACE
dc.subjectMODEL
dc.typeArticle
dc.date.updated2020-06-23T07:26:32Z
dc.contributor.departmentDEPT OF MICROBIOLOGY & IMMUNOLOGY
dc.description.doi10.1371/journal.pntd.0007610
dc.description.sourcetitlePLOS NEGLECTED TROPICAL DISEASES
dc.description.volume13
dc.description.issue8
dc.published.statePublished
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