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Title: Alloimmunogenicity of mesenchymal stem cell and its differentiated osteogenic lineage
Authors: LIU HUA
Keywords: Mesenchymal stem cell, allogenic, immune rejection, MHC, differentiation, rabbit
Issue Date: 29-Apr-2005
Citation: LIU HUA (2005-04-29). Alloimmunogenicity of mesenchymal stem cell and its differentiated osteogenic lineage. ScholarBank@NUS Repository.
Abstract: This study is to evaluate immunogenicity of allogenic mesenchymal stem cell (MSC) and its differentiated osteogenic cell (ost). MSC derived from New Zealand White rabbita??s iliac bone marrow was differentiated to osteogenic and chondrogenic lineages. These two lineages were confirmed by positive immunostaining and immunohistochemistry (ALP, Van Kossa, osteocalcin, osteonectin and osteopontin for osteogenic cells, and toluidine blue and collagen-II for chondrogenic cells). MHC molecules on MSC and ost were tested by flow cytometry. The immunogenicities of MSC and ost were tested by Mixed Lymphocyte Culture (MLC), Cell-Mediated Lympholysis (CML) and skin transplantation. Allogenic MLC and CTL against MSC and ost were similar to negative controls while significantly different from positive controls (p<0.05, ANOVA). No allogenic lymphocyte proliferation was observed in the stimulation by cellular secretion, and no accelerated allogenic skin rejections were observed in the allogenic MSC/ost presensitized rabbits. Thus, rabbit MSC and ost have been observed failing to elicit immunogenic response of allogenic lymphocytes.
Appears in Collections:Master's Theses (Open)

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