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Title: The role of cyclops/nodal signaling in zebrafish early embryogenesis
Authors: TIAN JING
Keywords: Nodal signaling, cyclops, Zebrafish, Temperature-sensitive mutation, Floor plate, pro-domain
Issue Date: 29-Sep-2006
Citation: TIAN JING (2006-09-29). The role of cyclops/nodal signaling in zebrafish early embryogenesis. ScholarBank@NUS Repository.
Abstract: Nodal proteins are members of the transforming growth factor-beta (TGF-b) family and have crucial roles in mesendoderm formation and left-right patterning during vertebrate development. We isolated a temperature-sensitive mutation affecting the zebrafish Nodal-related secreted signaling factor, Cyclops. I use this mutant allele, cycsg1, to answer a central question pertaining to the timing of vertebrate floor plate specification. I found that the floor plate in zebrafish is specified early in development, during gastrulation. I also revealed that the mechanism of the temperature-sensitive phenotype which caused by a premature stop codon in the pro-domain in cyclops gene is due to a readthrough but it is not stop-codon dependent. By detailed analysis in pro-domain of Cyclops, I found sequences important for protein activity, critical amino acid for inhibitor binding, and an important region responsible for the signaling range of Cyclops.
Appears in Collections:Ph.D Theses (Open)

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