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https://doi.org/10.1021/acs.analchem.6b00524
Title: | Real-Time Specific Light-Up Sensing of Transferrin Receptor: Image-Guided Photodynamic Ablation of Cancer Cells through Controlled Cytomennbrane Disintegration | Authors: | Zhang, Ruoyu Feng, Guangxue Zhang, Chong-Jing Cai, Xiaolei Cheng, Xiamin Liu, Bin |
Keywords: | Science & Technology Physical Sciences Chemistry, Analytical Chemistry AGGREGATION-INDUCED EMISSION THERAPEUTIC RESPONSES AIE CHARACTERISTICS TARGETED DELIVERY IN-SITU TURN-ON NANOPARTICLES APOPTOSIS PROBE PHOTOSENSITIZER |
Issue Date: | 3-May-2016 | Publisher: | AMER CHEMICAL SOC | Citation: | Zhang, Ruoyu, Feng, Guangxue, Zhang, Chong-Jing, Cai, Xiaolei, Cheng, Xiamin, Liu, Bin (2016-05-03). Real-Time Specific Light-Up Sensing of Transferrin Receptor: Image-Guided Photodynamic Ablation of Cancer Cells through Controlled Cytomennbrane Disintegration. ANALYTICAL CHEMISTRY 88 (9) : 4841-4848. ScholarBank@NUS Repository. https://doi.org/10.1021/acs.analchem.6b00524 | Abstract: | © 2016 American Chemical Society. Transferrin receptor (TfR) represents a unique target for specific imaging of cancer cells and targeted delivery of therapeutic reagents. Detection and qualification of TfR is thus of great importance for cancer diagnosis and therapy. In this contribution, a light-up probe TPETH-2T7 was developed by conjugating a red-emissive photosensitizer with aggregation-induced emission (AIE) characteristics to a TfR-targeting peptide T7. The probe is almost nonemissive by itself, but it gives turn-on fluorescence in the presence of TfR with a detection limit of 0.45 μg/mL. Cellular experiments show that the probe specifically binds to TfR-overexpressed cancer cells. Real-time imaging results reveal that the probe stains the MDA-MB-231 cell membrane in 30 min, which is followed by probe internalization. Experiments on image-guided photodynamic cancer ablation show that the therapeutic performance is better when TPETH-2T7 is localized on the cell membrane as compared to that being internalized into cells. Confocal laser scanning microscopy (CLSM) study reveals that cytomembrane disintegration allows quick ablation of MDA-MB-231 cells. | Source Title: | ANALYTICAL CHEMISTRY | URI: | https://scholarbank.nus.edu.sg/handle/10635/169772 | ISSN: | 00032700 15206882 |
DOI: | 10.1021/acs.analchem.6b00524 |
Appears in Collections: | Staff Publications Elements |
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