Please use this identifier to cite or link to this item: https://doi.org/10.1021/acs.analchem.6b00524
Title: Real-Time Specific Light-Up Sensing of Transferrin Receptor: Image-Guided Photodynamic Ablation of Cancer Cells through Controlled Cytomennbrane Disintegration
Authors: Zhang, Ruoyu 
Feng, Guangxue 
Zhang, Chong-Jing 
Cai, Xiaolei 
Cheng, Xiamin
Liu, Bin 
Keywords: Science & Technology
Physical Sciences
Chemistry, Analytical
Chemistry
AGGREGATION-INDUCED EMISSION
THERAPEUTIC RESPONSES
AIE CHARACTERISTICS
TARGETED DELIVERY
IN-SITU
TURN-ON
NANOPARTICLES
APOPTOSIS
PROBE
PHOTOSENSITIZER
Issue Date: 3-May-2016
Publisher: AMER CHEMICAL SOC
Citation: Zhang, Ruoyu, Feng, Guangxue, Zhang, Chong-Jing, Cai, Xiaolei, Cheng, Xiamin, Liu, Bin (2016-05-03). Real-Time Specific Light-Up Sensing of Transferrin Receptor: Image-Guided Photodynamic Ablation of Cancer Cells through Controlled Cytomennbrane Disintegration. ANALYTICAL CHEMISTRY 88 (9) : 4841-4848. ScholarBank@NUS Repository. https://doi.org/10.1021/acs.analchem.6b00524
Abstract: © 2016 American Chemical Society. Transferrin receptor (TfR) represents a unique target for specific imaging of cancer cells and targeted delivery of therapeutic reagents. Detection and qualification of TfR is thus of great importance for cancer diagnosis and therapy. In this contribution, a light-up probe TPETH-2T7 was developed by conjugating a red-emissive photosensitizer with aggregation-induced emission (AIE) characteristics to a TfR-targeting peptide T7. The probe is almost nonemissive by itself, but it gives turn-on fluorescence in the presence of TfR with a detection limit of 0.45 μg/mL. Cellular experiments show that the probe specifically binds to TfR-overexpressed cancer cells. Real-time imaging results reveal that the probe stains the MDA-MB-231 cell membrane in 30 min, which is followed by probe internalization. Experiments on image-guided photodynamic cancer ablation show that the therapeutic performance is better when TPETH-2T7 is localized on the cell membrane as compared to that being internalized into cells. Confocal laser scanning microscopy (CLSM) study reveals that cytomembrane disintegration allows quick ablation of MDA-MB-231 cells.
Source Title: ANALYTICAL CHEMISTRY
URI: https://scholarbank.nus.edu.sg/handle/10635/169772
ISSN: 00032700
15206882
DOI: 10.1021/acs.analchem.6b00524
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