Microarray analysis of host transcription in response to infection of arabidopsis thaliana protoplasts by coat protein mutants of TCV and HCRSV

Abstract

The interaction between Arabidopsis thaliana and Turnip crinkle virus (TCV), Hibiscus chlorotic ringspot virus (HCRSV) together with their mutants TCV-CPHCRSV and HCRSV-CPTCV was studied for the host genes involved in such process and the mechanism of host-virus interaction. The two coat protein (CP) mutants of TCV-CPHCRSV and HCRSV-CPTCV were obtained by substitution of CP genes, and their infectivity in Arabidopsis protoplast was confirmed by Northern blot analysis. Then Arabidopsis microarray analyses were used to detect host genes putatively involved in host-virus interactions by studying the transcript profiles of protoplasts transfected with TCV, HCRSV, TCV-CPHCRSV or HCRSV-CPTCV. Finally, many differentially expressed genes involved in transcription regulation, defense or stress response, phosphorylation, together with some unknown genes were obtained, and future studies on them may throw light on the mechanisms of interaction between host and virus. (134 words)