Please use this identifier to cite or link to this item: https://doi.org/10.1002/adfm.201902717
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dc.titleBright AIEgen-Protein Hybrid Nanocomposite for Deep and High-Resolution In Vivo Two-Photon Brain Imaging
dc.contributor.authorWang, Shaowei
dc.contributor.authorHu, Fang
dc.contributor.authorPan, Yutong
dc.contributor.authorNg, Lai Guan
dc.contributor.authorLiu, Bin
dc.date.accessioned2020-06-10T07:17:33Z
dc.date.available2020-06-10T07:17:33Z
dc.date.issued2019-07-01
dc.identifier.citationWang, Shaowei, Hu, Fang, Pan, Yutong, Ng, Lai Guan, Liu, Bin (2019-07-01). Bright AIEgen-Protein Hybrid Nanocomposite for Deep and High-Resolution In Vivo Two-Photon Brain Imaging. ADVANCED FUNCTIONAL MATERIALS 29 (29). ScholarBank@NUS Repository. https://doi.org/10.1002/adfm.201902717
dc.identifier.issn1616301X
dc.identifier.issn16163028
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/169621
dc.description.abstract© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Two-photon fluorescence imaging allows in vivo study of biological structures and activities in deep tissues, in which bright fluorophores with high photostability and good biocompatibility are highly desirable. Herein, a small-molecule fluorogen with aggregation-induced emission (AIEgen) is complexed with fetal bovine serum (FBS) proteins to develop a protein-sized AIEgen–protein hybrid nanocomposite (TPEPy-FBS) with bright far-red/near-infrared (NIR) emission, excellent photostability, and low phototoxicity for deep and high-resolution in vivo two-photon brain vasculature imaging. Upon complexation with FBS, the fluorescence of TPEPy is greatly intensified and a sixfold enhancement is observed with 10% FBS in aqueous media. The yielded TPEPy-FBS shows good physical stability in aqueous media and the phototoxicity of TPEPy is dramatically inhibited after complexation with FBS. Moreover, TPEPy-FBS exhibits bright two-photon fluorescence in far-red/NIR region and good photostability upon femtosecond laser excitation, which facilitates high performance in vivo imaging. A large imaging depth of 656 µm is obtained in brain vasculature network imaging with a high signal-to-background ratio of 234, where a small blood capillary of 1.05 µm can be resolved at an imaging depth of 656 µm. Highlighted is a simple and versatile strategy to develop efficient two-photon probes for in vivo biological imaging.
dc.language.isoen
dc.publisherWILEY-V C H VERLAG GMBH
dc.sourceElements
dc.subjectScience & Technology
dc.subjectPhysical Sciences
dc.subjectTechnology
dc.subjectChemistry, Multidisciplinary
dc.subjectChemistry, Physical
dc.subjectNanoscience & Nanotechnology
dc.subjectMaterials Science, Multidisciplinary
dc.subjectPhysics, Applied
dc.subjectPhysics, Condensed Matter
dc.subjectChemistry
dc.subjectScience & Technology - Other Topics
dc.subjectMaterials Science
dc.subjectPhysics
dc.subjectaggregation-induced emission
dc.subjectbrain imaging
dc.subjectdeep tissue
dc.subjectfluorophore-proteins
dc.subjecttwo-photon probes
dc.subjectAGGREGATION-INDUCED EMISSION
dc.subjectPHOTODYNAMIC THERAPY
dc.subjectGOLD NANORODS
dc.subjectQUANTUM DOTS
dc.subjectNANOPARTICLES
dc.subjectMULTIPHOTON
dc.subjectALBUMIN
dc.subjectTISSUE
dc.subjectFLUOROPHORES
dc.subjectLUMINESCENCE
dc.typeArticle
dc.date.updated2020-06-10T06:21:15Z
dc.contributor.departmentDEPT OF CHEMICAL & BIOMOLECULAR ENGG
dc.description.doi10.1002/adfm.201902717
dc.description.sourcetitleADVANCED FUNCTIONAL MATERIALS
dc.description.volume29
dc.description.issue29
dc.published.statePublished
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