Please use this identifier to cite or link to this item: https://doi.org/10.1002/adfm.201902717
Title: Bright AIEgen-Protein Hybrid Nanocomposite for Deep and High-Resolution In Vivo Two-Photon Brain Imaging
Authors: Wang, Shaowei 
Hu, Fang
Pan, Yutong 
Ng, Lai Guan
Liu, Bin 
Keywords: Science & Technology
Physical Sciences
Technology
Chemistry, Multidisciplinary
Chemistry, Physical
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Physics, Applied
Physics, Condensed Matter
Chemistry
Science & Technology - Other Topics
Materials Science
Physics
aggregation-induced emission
brain imaging
deep tissue
fluorophore-proteins
two-photon probes
AGGREGATION-INDUCED EMISSION
PHOTODYNAMIC THERAPY
GOLD NANORODS
QUANTUM DOTS
NANOPARTICLES
MULTIPHOTON
ALBUMIN
TISSUE
FLUOROPHORES
LUMINESCENCE
Issue Date: 1-Jul-2019
Publisher: WILEY-V C H VERLAG GMBH
Citation: Wang, Shaowei, Hu, Fang, Pan, Yutong, Ng, Lai Guan, Liu, Bin (2019-07-01). Bright AIEgen-Protein Hybrid Nanocomposite for Deep and High-Resolution In Vivo Two-Photon Brain Imaging. ADVANCED FUNCTIONAL MATERIALS 29 (29). ScholarBank@NUS Repository. https://doi.org/10.1002/adfm.201902717
Abstract: © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Two-photon fluorescence imaging allows in vivo study of biological structures and activities in deep tissues, in which bright fluorophores with high photostability and good biocompatibility are highly desirable. Herein, a small-molecule fluorogen with aggregation-induced emission (AIEgen) is complexed with fetal bovine serum (FBS) proteins to develop a protein-sized AIEgen–protein hybrid nanocomposite (TPEPy-FBS) with bright far-red/near-infrared (NIR) emission, excellent photostability, and low phototoxicity for deep and high-resolution in vivo two-photon brain vasculature imaging. Upon complexation with FBS, the fluorescence of TPEPy is greatly intensified and a sixfold enhancement is observed with 10% FBS in aqueous media. The yielded TPEPy-FBS shows good physical stability in aqueous media and the phototoxicity of TPEPy is dramatically inhibited after complexation with FBS. Moreover, TPEPy-FBS exhibits bright two-photon fluorescence in far-red/NIR region and good photostability upon femtosecond laser excitation, which facilitates high performance in vivo imaging. A large imaging depth of 656 µm is obtained in brain vasculature network imaging with a high signal-to-background ratio of 234, where a small blood capillary of 1.05 µm can be resolved at an imaging depth of 656 µm. Highlighted is a simple and versatile strategy to develop efficient two-photon probes for in vivo biological imaging.
Source Title: ADVANCED FUNCTIONAL MATERIALS
URI: https://scholarbank.nus.edu.sg/handle/10635/169621
ISSN: 1616301X
16163028
DOI: 10.1002/adfm.201902717
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