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Title: MCTP1, A novel member of transmembrane C2 domain protein
Authors: WU CHUN
Keywords: MCTP1, transmembrane, C2 domain, calcium binding, neuroscience
Issue Date: 17-Aug-2009
Citation: WU CHUN (2009-08-17). MCTP1, A novel member of transmembrane C2 domain protein. ScholarBank@NUS Repository.
Abstract: C2 domains are Ca2+ and/or phospholipids binding domains, found in many signal transduction or membrane fusion proteins. MCTPs are a novel family of multiple C2 domains containing proteins with one or more transmembrane regions. In this study, with the help of the in-house produced anti rat MCTP1 antibody, we identified two isoforms of rat MCTP1 proteins and found that in the CNS they are expressed mainly in neurons and some oligodendrocytes. Furthermore, the results from TEM showed the existence of MCTP1 proteins at the presynaptic vesicles, mitochondrial membrane and the outermost layer of myelin sheath in the rat CNS which suggested their role in synaptic transmission and Ca2+ homeostasis in brain. Through Western blots, we also demonstrated their distinct distribution pattern in different tissues and regulated expression profile during rat brain and muscle development. In addition, our preliminary result from overexpression showed a formation of aggregate-like particles in SHEP cells, suggesting a possible relationship between MCTP1 and neurodegenerative diseases. More studies are necessary to better understand the detailed MCTP1b s function in vertebrates and in relation to synaptic transmission, Ca2+ homeostasis and neurodegenerative diseases.
Appears in Collections:Master's Theses (Open)

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