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Please use this identifier to cite or link to this item: https://doi.org/10.21769/bioprotoc.2095
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dc.titleDot Blot Analysis of N6-methyladenosine RNA Modification Levels
dc.contributor.authorShen, Lisha
dc.contributor.authorLIANG ZHE
dc.contributor.authorYU HAO
dc.date.accessioned2020-06-02T00:56:58Z
dc.date.available2020-06-02T00:56:58Z
dc.date.issued2017-01-05
dc.identifier.citationShen, Lisha, LIANG ZHE, YU HAO (2017-01-05). Dot Blot Analysis of N6-methyladenosine RNA Modification Levels. BIO-PROTOCOL 7 (1). ScholarBank@NUS Repository. https://doi.org/10.21769/bioprotoc.2095
dc.identifier.issn2331-8325
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/168911
dc.description.abstractN6-methyladenosine (m6A) is the most prevalent internal modification of eukaryotic messenger RNA (mRNA). The total amount of m6A can be detected by several methods, such as dot blot analysis using specific m6A antibodies and quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) (Fu et al., 2014; Shen et al., 2016). Here we describe the method for fast detection of total m6A levels in mRNA by dot blot analysis using a specific m6A antibody.
dc.publisherBio-Protocol, LLC
dc.sourceElements
dc.subjectDot blot
dc.subjectRNA modification
dc.subjectm6A
dc.typeArticle
dc.date.updated2020-06-01T10:02:19Z
dc.contributor.departmentDEPT OF BIOLOGICAL SCIENCES
dc.description.doi10.21769/bioprotoc.2095
dc.description.sourcetitleBIO-PROTOCOL
dc.description.volume7
dc.description.issue1
dc.published.statePublished
dc.grant.idMOE2015-T2-1-002
dc.grant.idNRF-NRFI2016-02
dc.grant.fundingagencyMinistry of Education-Singapore
dc.grant.fundingagencySingapore National Research Foundation Investigatorship Programme
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